G-B-R复配物对模拟微重力效应诱导骨丢失的防护作用

The protective effect of G-B-R compound on bone loss induced by simulated microgravity

目的从天然产物中寻找到对微重力效应诱导骨丢失具有防护作用的复配物。方法通过24种天然产物对骨髓干细胞和成骨细胞的增殖活性,筛选出具有较强效果的天然产物并对其进行复配;随后通过复配物对模拟微重力效应下昆明小鼠骨质丢失的影响来评价其骨丢失防护作用。结果银杏叶和荞麦花粉的复配物显示出最强的骨髓干细胞增殖活性;熟地黄显示出最强的成骨细胞增殖活性;在模拟微重力环境下不同剂量的银杏叶、荞麦花粉与熟地黄的复配物均显示出不同程度的骨丢失防护作用;高剂量组可以显著提高模型组小鼠血清中碱性磷酸酶的活性及股骨中的有机物含量。结论通过对天然产物促骨髓干细胞和成骨细胞增殖活性进行筛选和复配,发现所获得的G-B-R复配物对微重力效应诱导的骨丢失具有良好的防护作用。

Objective To develop a potent bone loss protective compound from natural products under microgravity environment. Methods Twenty-four natural products were studied. The compound prescription was constituted with natural product which had high proliferative activity in bone marrow mesenchymal stem cells or osteoblasts. The effective products were screened and combined. The effect of the compound prescription on bone loss induced by simulated microgravity was examined in Kunming mice. Results Compound prescription of Ginkgo-Buckwheat Bee Pollen showed the strongest proliferation activity by bone marrow stem cells. Rehmannia performed the strongest proliferation activity by osteoblasts. Under simulated microgravity environment, different doses of G-B-R compound prescription showed different level of protective effect on bone loss. High dose of the compound increased the activity of ALP and the content of organics in the femur in model mice significantly. Conclusion G- B-R compound prescription, which is found and combined through screening from high proliferation by bone marrow stem cells and osteoblasts, has protective effect on bone loss induced by simulated microgravity.