星点设计-效应面法(CCD-RSM)优选颈痛巴布剂提取工艺研究

Study on Extraction Technology of Babu Agent for Neck Pain with CCD-RSM

目的优选颈痛巴布剂中葛根素和干膏得率的水回流提取工艺条件。方法以君药葛根中有效成分葛根素的提取量和干膏得率,作为考察指标,以星点设计-效应面法进行工艺优。结果优选工艺为加8.7倍水,提取3次,每次1.85h。结论优选工艺所得葛根素含量提取率高,干膏得率较高,工艺稳定可行,可以进行中试生产。

Objective To optimize the extraction process of puerarin in Babu agent for neck pain of extract. Methods The extraction amount of puerarin and the yield of extract were selected as the CCD-RSM was conducted to predict the optimal extraction process. Results The optimal conditions of extraction process were determined as follows:adding 8.7 times of water,refluxing 3times,1.85 h for each time. Conclusion Under the optimal conditions,the extraction rate of puerarin was higher,the yield of extract is higher,the optimized extraction technology was stable,feasible and suitable for pilot scale test.