基于PGF_(2α)浓度变化研究当归川芎对抗原发性痛经小鼠子宫组织痉挛的机制

Study of Angelica- Ligusticum Wallichii against Uterine Tissue Spasm of Primary Dysmenorrhea Mouse Based on PGF_(2α) Concentration Changes

目的从PGF2α浓度变化出发,研究当归-川芎对原发性痛经小鼠子宫组织痉挛的作用及其机制;方法采用雌二醇联合缩宫素建立原发性痛经(primary dysmenorrhea,PD)模型,将当归:川芎不同比例(1∶1、2∶1、3∶1、3∶2)作用于PD小鼠,观察小鼠扭体反应潜伏期及30min内扭体次数,用ELISA法测定小鼠子宫组织中前列腺素F2α(PGF2α)、前列腺素E2(PGE2)、孕酮(P)浓度变化;结果当归-川芎4种配比均能提高扭体潜伏期,减少扭体次数(P<0.05、0.01);均能降低PGF2α的浓度(P<0.01),提高PGE2及P的水平(P<0.05、0.01);PGF2α浓度与扭体次数呈正相关(P<0.01),与扭体反应潜伏期呈负相关(P<0.05);PGE2、P与扭体次数及扭体潜伏期之间无相关性(P>0.05);PGF2α与PGE2、P均呈现出负相关(P<0.05、0.01);结论当归-川芎能对抗PD小鼠子宫平滑肌痉挛,其机制与抑制PGF2α生成,提高PGE2、P水平相关。

Objective： To study the function and mechanism of Angelica- Ligusticum wallichii for uterine tissue spasm of primary dysmenorrhea mice on the base of PGF concentration changes. Methods ： Primary dysmenorrhea （PD） mouse model was established by using estradiol and oxytocin. Angelica -Ligusticum wallichii were put in different proportions （1： 1,2： 1, 3： 1, 3：2） in PD mice to observe writhing response latency and writhing times within 30 minutes and to measure prostaglandin F2α （ PGF2α ） , prostaglandin E2 （ PGE2 ） , progesterone （P） concentration of the mouse uterine tissue by ELLSA. Result： 4 kinds of ratio of Angelica - Ligusticum walliehii could increase writhing latency and reduce writhing times （ P 〈 0. 05, 0. 01 ） and also reduce the concentration of PGF2= （ P 〈 0. 01 ） and increase the level of PGE2 and P （ P 〈 0. 05, 0. 01 ）. PGF2α concentration was positively correlated with the writhing times （ P 〈 0.01 ） and negative- ly correlated with the writhing response latency （ P 〈 0. 05 ）. PGE2 and P had no eorrelation with writhing times and latency （ P 〉 0. 05 ）. PGF2α showed a negative correlation with PGE2 and P （P 〈 0. 05, 0. 01 ）. Conclusion： Angelica - Ligusticum wallichii can fight against uterine smooth muscle spasm of PD mice and its mechanism and inhibition of PGF2α production are correlated with an increase of PGEz and P levels.