复方大黄软膏中黄芩苷的含量测定及体外透皮特性

Study on the Content and Percutaneous Permeation Characteristic in Vitro of Baicalin in Compound Rhubarb Ointment

的：建立复方大黄软膏中黄芩苷的含量测定方法，并考察其体外透皮特性。方法：采用HPLC法测定黄芩苷含量，色谱柱为Agilenn200-C18柱（4．6mm×250mm，5μm），流动相为甲醇-水-磷酸（47：53：0．2），流速为1．0mL／min，检测波长为280nm；应用药物透皮扩散试验法考察其体外透皮特性。结果：黄芩苷的质量浓度在1．5172～242．75μg/L范围内与峰面积呈良好线性关系（r=1），平均加样回收率102．1％（RSD％=2．7％，n=6）；2批次复方大黄软膏中黄芩苷的含量分别为2．31mg／g、2．17mg／g，经离体裸小鼠皮肤24h平均累积透过率仅为0．25％。结论：该方法简便、快速、准确，可用于复方大黄软膏的质量控制及透皮特性评价。

Objective： To establish the method for the content determination of baiealin in compound rhubarb ointment, and to investigate its percutaneous permeation characteristic in vitro. Methods： The content of baicalin was determined by HPLC. The determination was performed on Agilent1200-C18 （4.6 mm×250 mm,5 μm） column with mobile phase consisted of methanol- water- phosphoric acid（47：53：0.2） at the flow rate of 1.0mL/min. The detection wavelength was set at 280 nm. The pereutaneous permeation characteristic in vitro was observed by the method of drug transdermal diffusion test. Results： The linear range of baicalin was 1.5172-242.75 μg/mL （r=l） with an average recovery of 102.1%（RSD%=2.7%,n=6）. The average content of baicalin in two batches compound rhubarb ointment were 2.31 mg/g and 2.17 rag/g, respectively. The average cumulative penetration rate of baicalin in 24 h was only 0.25%. Conclusion： The method is proved to be simple, rapid, and accurate, and can be used for the quality control of compound rhubarb ointment and percutaneous permeation characteristics research.