摘要
目的:优化丹参的乙醇提取工艺。方法:采用HPLC法检测丹参酮ⅡA的含量,同时以丹参酮ⅡA为指标,采用响应面法对乙醇浓度、提取时间、料液比3个影响因素进行优化。结果:建立了丹参乙醇提取模型,回归模型显著;提取优化条件为:乙醇浓度86.00%,提取时间44 min,料液比1∶17。结论:该提取工艺合理,丹参酮ⅡA的提取率高。
Objective： To optimize the extraction technology of Salviae Miltiorrhizae in alcohol. Methods： The content of tanshinone IIA was determined by HPLC. Concentration of alcohol, extraction duration and solid- liquid ratio were investigated by response surface methodology using the yield of tanshinone ⅡA as index.Results： The yield model for extracting tanshinone ⅡA in salviae mihiorrhizae was build, and the model is remarkable. The best conditions gained that the concentration of alcohol was 86%, the distilled time was 44 min, and that the solid-liquid ratio was 1：17. Conclusion： The extraction technology is reasonable, and there was high extraction rate of tanshinone ⅡA.
出处
《中医药导报》
2015年第23期43-46,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省中医药管理局科研项目(201402)
湖南省科技厅科技项目(2013SK3212)
长沙市科技局科技项目(K12ZD010-33)
关键词
丹参
丹参酮ⅡA
响应面法
提取工艺
优化
Salviae Miltiorrhizae Radix et Rhizoma
Tanshinone ⅡA
response surface methodology
extraction technology
optimization
