HPLC法测定养肾丸中大黄素的含量

Determination of Emodin in Yangshnen Pill(养肾丸) by HPLC

目的:建立养肾丸中大黄素含量的测定方法,为提高该制剂的质量标准提供研究依据。方法:采用HPLC法,动相:甲醇-0.1%磷酸(82∶18);色谱柱:Hedera Lichrospher柱(4.6 mm×250 mm,5μm);流速为0.8 m L/min,检测波长为254 nm,柱温为40℃;进样量:10μL。结果:淫羊藿苷在5.5μg^88μg/m L范围内线性关系良好,平均回收率为98.25%,RSD%为1.5%。结论:该方法简便、可靠、重现性好,可用于养肾丸的质量控制。

Objective： To establish a method for the determination of emodin in Yangshnen Pill, in order to provide envi- dence of research for quality standard. Methods： A HPLC method was used with Hedera Lichrospher column （250 mm×4.6 mm, 5 μm） and a mobile phase of Methanol -0.1%Phosphoric acid （82：18）. Flow rate was 0.8 mL/min,and column temperature was 40 ℃,with the detection wavelength at 254 nm as well as sample loop volume being 10 μL. Results： The calibration curve was linear within the range of 5.5 μg-88 μg/mL of emodin, and the average recovery was 98.25% （RSD=1.5%）. Conclusion： The method is simple, accurate and repeatable, which can be used for the quality control of Yangshnen Pill.