实验兔肾动脉使用医用胶栓塞的可行性研究

Feasibility study of embolization with medical adhesive on renal artery in rabbits

目的通过动物实验观察医用胶栓塞实验兔肾动脉的可行性及安全性,探讨不同栓塞剂浓度对血管栓塞过程及效果的影响,为其临床应用提供指导。方法用不同比例的超液化碘油与医用胶组成的混悬液对18只实验兔肾动脉进行栓塞,观察栓塞情况及效果。结果栓塞剂经导管注射顺利,可经微导管行反复栓塞,安全有效,栓塞可靠,均未见血管再通和造影剂外溢。术后病理检查HE染色后光学显微镜下见动脉内栓塞剂呈不规则形或分支状折光性透亮物质,其中,5∶1、4∶1组以细、小动脉栓塞为主,二者比较差异无统计学意义(P>0.05),3∶1组以中型动脉栓塞为主,与5∶1组、4∶1组比较差异有统计学意义(P<0.05),肾组织可见充血、变性、凝固性坏死、钙化及纤维组织增生等不同改变。术后肾功能呈一过性损害,白细胞一过性升高,1周后均恢复至术前水平,术后肝功能未见明显异常。结论医用胶栓塞实验兔肾动脉可行,不同浓度的医用胶可以选择性栓塞肾动脉各级分支。

Objective To observe the feasibility and security of the embolization with medical adhesive on renal artery in ran- bits by animal experiments,and explore the effectiveness of different concentration of embolic agents on the process and the effect of embolization for vascular,and provide guidance for clinical application of medical adhesive. Methods A mixture of different ratios of lipiodol and medical adhesive were used to embolize the renal artery in 18 rabbits, the usage and effects of embolization were ob- served. Results Embolic agents were seen under the fluoroscopy,easy to control and inject transcatheter,also repeated injected by one micro-catheter safety and effectively. Angiography review showed a reliable embolization, no vascular recanalization and no spillover of contrast agent. Optical microscope, after lie staining in postoperative pathological examination , showed irregular shaped embolic agents or branch like refractive index of transparent material in the embolized artery. In 5 ： 1 group and 4 ： 1 group,arteriole and small artery were mainly embolized,there was no significant difference between the two groups（P〉0.05） ;Whereas medium sized artery was mainly embolized in 3 ： 1 group, compared with the 5 ： 1 group and 4 ：1 group,the differences was statistically significant（P〈0.05）. After operation, renal function was transient damage, leukocyte was transient increasing, one week later, both of renal function and leukocyte were recovered,and there was no significantly renal function anomaly. Conclusion It is feasible,safe and effective for medical adhesive＇ to embolize rabbits renal artery, the different concentration of the embolic agents can embolize the different branches of rabbit renal artery.