摘要
2014年在河南省郑州、济源、新郑、中牟、扶沟等地发现大量保护地番茄植株的叶脉间出现褪绿症状,疑似由番茄褪绿病毒(Tomato chlorosis virus,To CV)侵染引起。以这5个地区的番茄病叶为样本提取总RNA,利用ToCV HSP70h(Heat shock protein 70 homolog,热激蛋白70家族)基因的特异引物对样本进行RT-PCR检测,均扩增出约460bp大小的目的条带。PCR产物测序和序列分析结果表明,该序列与国际上报道的ToCV HSP70h的序列相似性达到99%以上,表明ToCV是为害河南省番茄产区造成褪绿症状的主要病原之一。
In 2014, severe interveinal chlorosis of typical Tomato chlorosis virus Tomato chlorosis virus （ ToCV ） symptom infections were observed in greenhouse tomato plants in Zhengzhou, Jiyuan, Xinzheng, Zhongmou and Fugou region of Henan Province. The disease samples from these 5 regions were collected, and a Reverse Transcript-PCR assay was used to detect the virus with total RNA isolated from the disease samples by specific primers of ToCV HSP7Oh. About 460 bp PCR product was obtained. The PCR products were sequenced and analyzed. The result showed that they had the highest identity （ 〉 99% ） with that of ToCV. This result also indicated that ToCV is one of the main chlorosis disease pathogen in tomato of Henan Province.
出处
《中国蔬菜》
北大核心
2015年第12期25-28,共4页
China Vegetables
基金
河南省现代农业产业技术体系建设专项(S2010-03-G02)
关键词
河南省
番茄褪绿病毒
RT-PCR
分子鉴定
Henan Province
Tomato chlorosis virus
RT-PCR
Molecular identification
