可视化核酸恒温扩增技术在日本血吸虫病筛查和诊断中的应用

Application of Visible Isothermal Nucleic Acid Amplification Technology in Screening and Diagnosis of Schistosomiasis Japonicum

日本血吸虫病的筛查和诊断在血吸虫病的防治中处于重要的地位,而核酸诊断是其潜在的优良检测方法。拟建立一套完整的"可视化日本血吸虫核酸恒温扩增检测试剂",用于基层血防单位对日本血吸虫病的筛查和诊断等工作。用腹壁拔毛贴片法给新西兰大白兔感染日本血吸虫,用多种核酸提取方法抽提其血清或血浆中的基因组DNA,并用"可视化日本血吸虫核酸恒温扩增检测试剂"对其进行检测。结果表明,该研究所设计的检测方法可以方便地检测到100条日本血吸虫尾蚴感染的新西兰大白兔,说明可视化核酸恒温扩增技术在日本血吸虫病的筛查和诊断中具有潜在的应用可能性。

A complete system of＂visible isothermal nucleic acid amplification reagent＂was developed and used for the prevention and control of schistosomiasis in the basic units.In this work,New Zealand white rabbits were infected with Schistosoma japonicum cercariae by using sticking patch on plucked abdomen,and then,genomic DNA of serum or plasma was extracted by the methods of nucleic acid extraction,and detected with＂visible isothermal nucleic acid amplification reagent＂.The results revealed that the test method could conveniently detect white rabbits infected with 100 cercariae of Schistosoma japonicum.Moreover,it also illustrated that the visible isothermal nucleic acid amplification technology has important application value to screening and diagnosing schistosomiasis.