披针叶黄华碱对奶牛子宫内膜炎病原菌增殖的影响

Effect of Thermopsis lanceolata Alkaloids on Pathogenic Bacteria Prolifeeration of Dairy Cow Endometritis

为研究披针叶黄华碱对奶牛产后子宫内膜炎致病菌的体外抗菌活性,采集宁夏地区20头确诊为子宫内膜炎的患牛子宫黏液,通过有氧条件和厌氧条件培养,依据细菌形态学和生化特性进行分离鉴定。利用披针叶黄华碱中的总碱、黄华碱、四氢脱氧阿艮亭碱、金雀花碱进行药物敏感试验,以试管双倍稀释法测定其最小抑菌浓度(MIC)。结果表明,患牛细菌感染率为100%,病料分离细菌68株,其中大肠埃希菌占55.8%,溶血性链球菌占20.5%,非溶血性链球菌占14.7%,沙门菌占9.0%。披针叶黄华总碱、黄华碱、四氢脱氧阿艮亭碱对大肠埃希菌、溶血性链球菌、非溶血性链球菌、沙门菌均有一定的抗菌活性,其中最为敏感的是非溶血性链球菌,MIC为15.63 mg/mL^62.5 mg/mL,其次为沙门菌,MIC为31.25 mg/mL^125mg/mL,大肠埃希菌的MIC为62.5.25mg/mL^250mg/mL,溶血性链球菌的MIC为125mg/mL^500mg/mL,金雀花碱则对以上4种细菌无抗菌活性。

To understand effects of Thermopsis lanceolata alkaloid on pathogenic bacteria proliferation in vitro of Dairy Cow Endometritis,20 samples of uterus secretions from 20 dairy cows with clinical puerperal metritis were adopted to isolate and identify pathogenic bacteria by the morphological and biochemical characteristics of bacteria and the aerobic and anaerobic cultivation.The results showed that infection rate was100%,totally 68 strains of 4species of pathogenic bacteria were isolated and identified,including hemolytic Streptococcus 20.5%,non-hemolytic Streptococcus 14.7%,E.coli 55.8%,Salmonella 9.0%.The minimum inhibitory concentration（MIC）wasused to determine the antibacterial activities.The results showed that total alkaloids of Thermopsis lanceolata,thermopsine,argintine had similar antibacterial activities,non-hemolytic Streptococcus was most sensitive,with MIC of 15.63mg/mL-62.5mg/mL,followed by Salmonella,with MIC of 31.25mg/mL-125mg/mL,E.coli with MIC of 62.5.25mg/mL-250mg/mL,hemolytic Streptococcus with MIC of 125mg/mL-500mg/mL.Sparteine has no antibacterial activity.