朱砂七多糖对雏鸡十二指肠组织形态的影响

Effects of Polygonum cillinerve Polysaccharides on Duodenum Histology of Chickens

通过在雏鸡饮水中每天添加低(0.3g/kg)、中(0.5g/kg)、高(0.7g/kg)剂量的朱砂七多糖(Polygonum cillinerve polysaccharide,PCP),运用常规组织学制片技术和染色方法分别观察了给药后7、14、21d鸡十二指肠的组织学特点,肠壁黏膜层上皮内淋巴细胞(intraepithelial lymphocytes,IEL)、柱状上皮细胞间杯状细胞(goblet cells,GC)和肠壁结缔组织内肥大细胞(mast cells MC)的数量差异,研究了朱砂七多糖对雏鸡十二指肠组织形态的影响。结果表明,PCP可使雏鸡十二指肠的绒毛高度增加、隐窝深度降低、绒毛高度与隐窝深度比值加大;并可增加黏膜上皮内淋巴细胞和杯状细胞以及肠壁肥大细胞的数量,表明PCP具有促进雏鸡十二指肠组织发育及增强肠道黏膜免疫的作用。

To evaluate the effect of Polygonum cillinerve polysaccharides（PCP）on duodenum histology,the chickens were administrated with low dose（0.3g/kg）,middle dose（0.5g/kg）,and high dose（0.7g/kg）of PCP by drinking water supplementation respectively,then the histological structure of duodenum,distribution of both intraepithelial lymphocytes（IEL）,goblet cells in mucosa and mast cells in whole intestinal wall were observed by using common histological technologies on days 7,14 and 21after the PCP treatment.The results showed that PCP could increase the villum height（VH）and the ratio of villum height to crypt depth（VH/CD）,and decrease the crypt depth（CD）.In addition,PCP could increase the number of intraepithelial lymphocytes（IEL）,goblet cells（GC）in mucosa and mast cells（MC）in whole intestinal wall.The results indicated that PCP possessed the role of prompting duodenum development and enhancing intestinal mucosal immunity in chickens.