浒苔多糖体外抗猪传染性胃肠炎病毒研究

Study on Anti-TGEV of Enteromorpha Polysaccharide in Vitro

为研究浒苔多糖体外抗猪传染性胃肠炎病毒(Transmissible gastroenteritis virus,TGEV)的效果,用水煮醇沉法从浒苔中提取浒苔多糖粗品,细胞维持液倍比稀释后,以猪睾丸细胞(ST)为细胞模型,采用细胞病变效应(CPE)试验,通过直接杀灭、抑制病毒复制和阻断病毒吸附3个方面进行研究。结果表明,浒苔多糖对ST细胞最大安全浓度为1.57mg/mL;浒苔多糖在体外对TGEV作用2h的最小杀灭浓度为0.2mg/mL,作用4h的最小杀灭浓度为0.1mg/mL;浒苔多糖浓度越高对TGEV复制的抑制作用越明显,TGEV感染细胞时间越长,对TGEV复制的抑制作用越低,抑制TGEV复制的最小浓度为1.57mg/mL;浒苔多糖对TGEV吸附ST细胞阻断作用的最小浓度与浒苔多糖作用于ST细胞的时间有关,2h为0.393mg/mL,4h为0.2mg/mL。浒苔多糖在体外对TGEV具有直接杀灭和阻断吸附的作用,为浒苔多糖抗病毒功能提供了理论依据。

The crude extraction of Enteromorpha polysaccharide was used to study the inhibitory effect of TGEVin vitro on ST cells.Enteromorpha was processed by decocting with water and precipitating with ethanol successively,and the crude Enteromorphapolysaccharide was abtained.CPE assay was conducted to explore the antiviral mechanism of the extraction of Eteromorpha polysaccharide through the following experiments,including the directly viruscidal test,virus replication inhibition test,and the test of blocking absorbtion of the virus to cells.The results showed that the maximum of safety concentration of Enteromorpha polysaccharide to ST cells was 1.57mg/mL;the minimum concentration of Enteromorphapolysaccharide directed anti-TGEVin vitro was 0.2mg/mL for 2h,0.1mg/mL for 4h.Higher concentration of Enteromorpha polysaccharide showed stronger inhibition to TGEV replication.The longer time TGEV infected cell,the less ability of inhibiting TGEV replication were got.The minimum inhibition concentration of Enteromorphapolysaccharide to TGEV replication was related to the action time of Enteromorphapolysaccharide on ST cells,as 1.57mg/mL for 1h,0.393mg/mL for 2h,0.2mg/mL for 4h.This study indicated that Enteromorphapolysaccha has a strong anti-TGEV ability in vitro,by dircectly virucidal effect and inhibiting the adsorption of virus to cells.