迈瑞EH-2080B全自动尿沉渣分析系统与手工镜检结果的比较

Comparison on the results of Mindray EH-2080B fully automatic urinary sediment analysis system made in China and manual microscopy

目的对迈瑞EH-2080B全自动尿沉渣分析系统(简称EH-2080B)的定量分析检测结果进行比对,并对其功能扩展进行验证。方法收集门诊、住院患者2 243份新鲜尿液(1 h内)标本,采用EH-2080B检测,以传统的手工镜检作为标准方法对其做评估。利用假阳(阴)性率与人工镜检结果进行定性比对;选取患者红细胞(RBC)、白细胞(WBC)阳性的新鲜尿液标本100例进行RBC、WBC定量比对;利用目视低倍单视野对充液静置时间段和利用视觉暂留对低倍拍照和高倍拍照转换视野瞬间人工浏览屏幕视野,对可能活精子以及阴道毛滴虫补充动态观察进行功能扩展评价。结果 EH-2080B批内精密度及批间精密度均符合要求[WBC和RBC均<10%,上皮细胞(EC)<30%];与手工镜检法相关性较好[RBC:相关系数(r)=0.973,P=0.000;WBC:r=0.990,P=0.000;EC:r=0.959,P=0.000]。与人工镜检法的比对结果显示,WBC、RBC、真菌等均存在一定程度的假阳(阴)性率,但结合干化学分析、拍照信息与自动分析结果进行综合分析,临床尿液标本随机抽样的总体符合率在85%以上且RBC、WBC阳性的新鲜尿液标本RBC、WBC定量比对差异无统计学意义(P>0.05);人工浏览视野对可能活精子以及阴道毛滴虫补充动态观察可以捕捉到动态信息或可疑动态信息。结论迈瑞EH-2080B全自动尿沉渣分析系统的结果与手工镜检基本相符,对尿有形成分有一定的识别分析能力,但在实际工作中特殊标本仍需人工镜检识别确认。利用不同方法检测结果的互补作用,既可提高工作效率,也有助于提高检测质量。

Objective To compare the resuhs of quantitative analysis of Mindray EH-2080B fully automatic urinary sediment analysis system made in China （ EH-2080B ）, and to verify its function expansion. Methods A total of 2 243 fresh urine samples （within lh） from outpatients and inpatients were analyzed by traditional manual microscopy as a standard method, and the system was evaluated. The qualitative comparison between EH-2OSOB and manual microscopy results was evaluated by false positive （negative） rate. The 100 fresh urine samples with positive red blood cell （RBC） and white blood cell （WBC） were selected to do quantitative comparison of RBC and WBC. While low power single view of liquid filling static time period and visual persistence of low power and high power photographed conversion instant view were used to observe, living sperms and trichomonas vaginalis＇ dynamic observation messages by the artificial screen browsing were useful to evaluate the function extension of the instrument. Results The within-run and between- run preeisions of EH-2080B met the requirements [ WBC 〈 10%, RBC 〈 10%, epithelial cell （EC） 〈 30% ]. The correlation with manual microscopy was good [ correlation coefficient （r） for RBC = 0. 973, P = 0. 000 ; r for WBC = 0.990, P = 0.000 ; r for EC = 0.959, P = 0. 000 ]. WBC, RBC, fungus and so on were of a certain degree of false positive （negative） rate, but the comprehensive analysis of eombinating dry chemistry information, photo information and automatic urinary sediment analysis was reliable. The coincidence rate by random sampling was more than 85% ,and the RBC and WBC quantitative comparison difference fi＇om fresh urine samples with RBC and WBC positivities had no statistical significance （ P 〉 0.05 ）. The artificial browsing could be added to capture the dynamic information or suspicious dynamic information of living sperms and Trichomonas vaginalis＇ dynamic observation. Conclusions The results of EH-2080B and manual microscopy are consistent. EH-2080B has some recognition analysis tbr urine formed elements, but for the actual work or some special specimens, manual microscopy identification is needed. The complementary effect of different detection methods can improve the work efficiency and improve the detection quality.