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新疆南疆牛乳头状瘤病毒鉴定与基因分型 被引量:7

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摘要 为探究新疆南疆某奶牛场疑似感染牛乳头状瘤病及病毒基因型,本试验采取牛皮肤病变肿瘤样组织,进行组织病理学检查,并提取病料DNA,以乳头状瘤病毒(bovine papillomavirus,BPV)L1基因简并引物和通用引物(FAP59/FAP64和MY11/MY09)进行PCR扩增、测序、序列分析及遗传进化树构建。测序结果表明:新疆南疆Aks-01株与Gen Bank收录的BPV-2基因型参考株核苷酸序列比较,核苷酸同源性可达95%,为BPV-2基因型;与BPV-1、BPV-13基因型参考株遗传进化关系最近,同属Delta属。结合临床症状、组织病理学检查,确定该奶牛场牛乳头瘤由BPV-2基因型感染引起。
出处 《畜牧与兽医》 北大核心 2015年第11期99-103,共5页 Animal Husbandry & Veterinary Medicine
基金 国家自然科学基金(31160497 31060024) 塔里木大学校长基金项目(TDZKTG201402 TDZKCX201401) 塔里木大学高教研究项目(TDGJ1408)
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