铜(Ⅱ)离子对橙皮素清除自由基作用的影响

Study on effect of copper(Ⅱ) ion on radical scavenging of hesperetin

为探讨Cu2+存在对橙皮素(Hsp)清除自由基作用的影响.将Hsp与Cu2+混合即生成不稳定配合物(Hsp-Cu),利用UV-Vis光谱测定Hsp及Hsp-Cu对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)、超氧阴离子自由基(O2-·)的清除作用.在实验条件下Hsp-Cu对DPPH·及·OH有一定的清除作用,但清除能力不如Hsp.Hsp-Cu不能有效清除O2-·,且在邻苯三酚自氧化过程中表现出促氧化作用.表明Hsp作为抗氧化剂使用时,应避免与Cu2+接触,以免影响其抗氧化能力.

To study the affection of Copper （ II ） ions on radical scavenging of hesperetin . Unstable hesperetin -copper （ II ） complex was generated when hesperetin was mixed with copper （ II ） chloride dihydrate .By the contrast tests on the scavenging reaction to DPPH radical, hydroxyl radical （· OH）, and superoxide free radical （O2 -· ） of hesperetin -copper （ II） complex and hesperetin , proves that the scavenging activity of DPPH ＆#183; and ·OH of hesperetin-copper （ II） complex was weaker than that of hesperetin .The hesperetin-copper （II） complex can’t scavenge O2 -·; showing accelerated oxidation in the process of pyrogallol autoxidation .Hesperetin was used as an antioxidant , cannot coexist with Cu 2＋, so as not to affect the antioxidant capacity of hesperetin .