RP-HPLC法测定北五味子中6种木脂素类成分

Simultaneous determination of content of 6 kinds of lignans in Fructus schisandrae chinensis by RP-HPLC

采用RP-HPLC法同时测定北五味子中6种木脂素类成分.色谱柱为Agilent ZORBAX SBC18（5μm,4.6×150 mm）,流动相为甲醇-水溶液梯度洗脱,检测波长为254 nm,柱温为25℃.结果表明：五味子甲素在40.4~600.0μg/m L（r=0.999 8）、五味子乙素在42.0~420.0μg/m L（r=0.9999）、五味子丙素在15.8~118.8μg/m L（r=0.999 5）、五味子醇甲在10.0~160.0μg/m L（r=0.9999）、五味子醇乙在15.5~388.0μg/m L（r=0.999 5）、五味子酯甲在16.3~408.0μg/m L（r=0.999 9）范围内峰面积与对照品质量浓度呈良好的线性关系（n=6）.6种成分的加样回收率均高于98%,RSD值小于3%本方法简便,准确,快速,重现性好,可用于北五味子中有效成分的定量分析.

In this paper , RP-HPLC to simultaneously determine 6 kinds of lignans in fruc-tus schisandrae chinensis was adopted .The column was Agilent ZORBAX SB -C18 （5 μm, 4.6 ×150 mm）.The mobile phase was consisted of methanol -water solution（gradient elu-tion）.The detect wavelength was set at 254 nm, and the column temperature was 25 ℃. Results indicated that deoxyschizandrin at the range of 40.4~600.0μg/mL （r=0.999 8）,γ-Schisandrin at the range of 42.0~420.0 μg/mL （r=0.999 9）, Schisandrin C at the range of 15.8~118.8μg/mL （r=0.999 5）, Schisandrin at the range of 10.0~160.0μg/mL （r =0.999 9）, Schisandrol at the range of 15.5 ~388.0 μg/mL （ r =0.999 5） Schisantherin A at the range of 16.3~408.0 μg/mL （r=0.999 9） have good linear rela-tionship between peak area and concentration of reference substance （ n=6 ） .The sample recovery rate of 6 kinds of ingredients was higher than 98%, and the RSD value was less than 3%.This method was simple , accurate , celerity and good reproducibility , and can be used for quantitative analysis of effective ingredients of fructus schisandrae chinensis.