消银解毒袋煎剂的HPLC-DAD指纹图谱研究

Study on HPLC-DAD Fingerprint of Xiaoying Jiedu Decoction

目的:采用从成品图谱逆向回溯至原料药材的方式,研究消银解毒袋煎剂HPLC-DAD指纹图谱,建立成品整体特征的质量评价方法。方法:采用HPLC-DAD检测,色谱条件:Dikma Platisil ODS C18(250 mm×4.6 mm,5μm);流动相:乙腈-(0.1%磷酸-0.1%三乙胺溶液),梯度洗脱;柱温30℃。结果:建立的HPLC-DAD指纹图谱中,16个色谱峰分别归属到处方中黄芩、鱼腥草、枳壳、紫草、甘草5个药材,4个色谱峰可明确化学成分。结论:建立的HPLC-DAD指纹图谱表达了消银解毒袋煎剂可测组分的整体特征,提高了对中药复方质量控制的专属性,逆向回溯的研究模式扩展了大复方质量研究的思路。

Objective： Based on backtracking method,identification and quality evaluation of Xiaoying Jiedu Decoction（ XYJDD） by HPLC fingerprint analysis were carried out. Methods： HPLC-DAD fingerprint of XYJDD was conducted with Dikma Platisil ODS C18column（ 250 mm × 4. 6 mm,5 μm） and gradient elution with the mobile phase consisting of acetonitrile-（ 0. 1% phosphoric acid-0. 1% triethylamine solution） at column temperature of 30 ℃. On the basis of the established chromatographic pattern of XYJDD,tracking backward to the corresponding crude herbal drugs in the formula and the attribution of most peaks in the XYJDD fingerprint can be figured out. Results： 16 peaks of XYJDD HPLC fingerprint were assigned by parallel comparison with the fingerprint of the five corresponding crude drugs in the formula of Scutellariae Radix,Glycyrrhizae Radix et Rhizoma,Houttuyniae Herba,Aurantii Fructus,Arnebiae Radix. Four peaks can be identified with the chemical reference substances. Conclusion： The entirety of XYJDD HPLC fingerprint enhances the specialty for control and assessment of the product quality,and the backtracking experimental method can be expected to be applied in chromatographic fingerprinting analysis of complex Chinese patent medicine.