壳聚糖修饰的托氟啶固体脂质纳米粒的制备

Preparation of Chitosan Modified TFu Solid Lipid Nanoparticles

目的:研究托氟啶固体脂质纳米粒及壳聚糖修饰的托氟啶固体脂质纳米粒的制备方法。方法:采用薄膜-超声分散法制备托氟啶固体纳米脂质粒(TFu-SLNs)及壳聚糖修饰的TFu-SLNs,并对纳米粒的形态、粒径和表面电位进行测定,通过单因素考察及正交设计优化制备方法同时考察处方稳定性。结果:薄膜-超声分散法制备的TFu-SLNs平均粒径为160.2 nm,Zeta电位为-33.12 mV,壳聚糖修饰TFu-SLNs平均粒径为400.3 nm,Zeta电位为+12.87 mV。经壳聚糖修饰后,随着壳聚糖浓度的增加,电位逐渐增大。优化后的处方重复性、稳定性良好。结论:通过采用正交设计法对TFu固体脂质纳米粒处方进行优化,得到TFu固体脂质纳米粒及壳聚糖修饰的TFu固体脂质纳米粒的优化处方。

Objective： To study the preparation methods of N3-O-toluyl-flulorouracil （TFu） solid lipid nanoparticles （SLNs） and chitosan modified TFu SLNs. Methods： TFu-SLNs and chitosan modifed TFu-SLNs were prepared by film dispersion-homogenization methods. The morphology, particle diameter and zeta potential were detected. The preparation methods were optimized by single factor experiments and an orthogonal design, and the stability of the nanopartieles was also studied. Results： The mean diameter of TFu- SLNs was 160.2nm, and the zeta potential was -33.2 inV. The mean diameter of ehitosan modified TFu- SLNs was 400.3nm, and the zeta potential was ＋ 12.87 mV. With the concentration increase of chitosan, the zeta potential was enhanced. The optimized TFu- SLNs had higher reproducibility and stability. Conclusion： The formula of TFu-SLNs is optimized by an orthogonal design to obtain the optimal formula of TFu -SLNs and ehitosan modified TFu-SLNs.