重楼根茎与须根中四种重楼皂苷含量比较

Comparative Study on Four Polyphyllins Content in Paridis Rhizome and Fibril

目的:比较重楼根茎与须根中四种重楼皂苷含量,考察皂苷类次生代谢产物在重楼不同生长部位的分布情况,为重楼药材综合开发利用提供参考。方法:采集武当山区及神农架林区的重楼药材样本,采用高效液相色谱法测定重楼根茎及其须根中主要次生代谢产物重楼皂苷Ⅰ、Ⅱ,Ⅵ,Ⅶ的含量。结果:须根中四种重楼皂苷总量明显高于根茎;薯蓣皂苷类成分在两个部位差异不显著,而偏诺皂苷类成分含量在须根中明显高于根茎。结论:从所含主要化学成分的角度分析,须根与根茎具有化学等同性,可以考虑入药用;但从重楼皂苷含量的角度分析,须根与根茎中分布有明显差异,有可能在须根中合成后转移至根茎中贮存。

Objective： To investigate the distribution of four polyphyllins in different parts of paris polyphylla by means of compa- ring the contents of relative constituents in paridis rhizome and fibril to provide reference for comprehensive exploitation and utilization of paris polyphylla. Methods： Paris polyphylla samples were collected from Wudang mountain area and Shennongjia forest area. The contents of main secondary metabolites including polyphillin Ⅰ,Ⅱ , Ⅵ and Ⅶ in paridis rhizome and fibril were evaluated by HPLC. Results： The four polyphyllins contents had obvious differences in different parts of polyphylla. The total content of four polyphillins in fibril was significantly higher than that in rhizome. Diosgenin compositions had no significant difference in the two parts, and the con- tents of pennogenin compositions in fibril showed significantly higher than those in rhizome. Conclusion： As for the main chemical com- positions contained in polyphylla, there is chemistry equality between fibril and rhizome, thus both of them can be used for medicine. However, as for the contents of four steroidal saponins, the distribution of secondary metabolites has obvious difference between fibril and rhizome, and the result may be caused by steroidal saponins transferred to rhizome for storage after the synthesis in fibril.