HPLC法测定抗601合剂中黄芩苷的含量

Determination of Baicalin in Kang 601 Mixture by HPLC

目的:建立抗601合剂中黄芩苷的含量测定方法。方法:采用高效液相色谱法。色谱柱为Agilent Eclipse Plus-C_(18).(250 mm×4.6 mm,5μm),流动相为甲醇:0.1%磷酸溶液(48:52),检测波长为280 nm,柱温为室温(25℃),流速为0.8ml·min^(-1),进样体积为20μl。结果:黄芩苷测定质量浓度在18.897~188.975μg·ml^(-1)范围内与其相应峰面积积分值呈良好线性关系(r=0.999 9);平均加样回收率为99.86%,RSD为0.32%(n=9)。结论:该方法专属性、重复性、准确性好,可用于抗601合剂中黄芩苷的含量测定。

Objective： To establish a method for the determination of baicalin in Kang 601 mixture. Methods： An HPLC method was adopted. An Agilent Eclipse Plus-C18 column （250 mm ×4.6 mm, 5 μm） was used at room temperature （25 ℃ ） and eluted by methanol-0.1% phosphoric acid solution（48： 52） with a flow rate of 0.8 ml min-1. The detection wavelength was 280 nm and the injection volume was 20 μl. Results： A good linear relationship was obtained between the peak area and the concentration of baicalin within the range of 18. 897-1 88.975 μg ml - 1（ r = 0.999 9）. The average recovery was 99.86% （ RSD = 0.32%, n = 9 ）. Conclu- sion： The established method is specific, accurate and reproducible, which can be used for the determination of baicalin in Kang 601 mixture.