小儿至宝丸中朱砂和雄黄的含量测定

Determination of Cinnabar and Realgar in Xiao'er Zhibao Pills

目的:建立小儿至宝丸中朱砂和雄黄的含量测定方法。方法:分别采用原子吸收分光光度法(AAS)和电感耦合等离子体发射光谱法(ICP-AES)测定小儿至宝丸朱砂中的汞和雄黄中的砷含量。结果:AAS法测定的汞的线性范围为5~100Rg·ml^(-1)(r=0.999 9),砷的线性范围为2~50μg·ml^(-1)(r=0.9992),汞的平均回收率为99.3%,RSD为3.0%(n=6),砷的平均回收率为104.5%,RSD为2.1%(n=6);ICP-AES法测定的汞的线性范围为0.1~20.0μg·ml^(-1)(r=0.99997),砷的线性范围为0.1-20.0μg·ml^(-1)(r=0.99999),汞的平均回收率为96.6%,RSD为1.4%(n=6),砷的平均回收率为104.1%,RSD为1.1%(n=6)。结论:本文建立的方法准确、专属性强,可用于控制小儿至宝丸中毒性成分的含量。

Objective： To establish a method for the determination of cinnabar and realgar in Xiao＇ er Zhibao pills. Methods： Mercury in cinnabar and arsenic in realgar in Xiao＇er Zhibao pills were determined respectively by atomic absorption spectrometry （AAS） and inductively coupled plasma atomic emission spectrometry（ICP-AES）. Results： A good linear correlation of mercury and arsenic was observed within the range of 5-100 μg ml-1（ r = 0.999 9） and 2-50 μg ml-1（ r = 0.999 2）, and the average recovery was 99.3% with RSD of 3.0% （n =6） and 104.5% with RSD of 2.1% （n =6） by AAS. A good linear correlation of mercury and arsenic was observed within the range of 0.1-20.0μg ml - 1（ r = 0.999 97 ） and 0.1-20.0 μg ml -1 （ r = 0.999 99 ）, respectively, and the average recovery was 96.6% with RSD of 1.4% （ n = 6 ） and 104.1% with RSD of 1.1% （ n = 6 ） by ICP-AES, respectively. Conclusion： The method is accurate and specific, and can be used for the quality control of Xiao＇er Zhibao pills.