顶空气相色谱法测定怀氏液中乙醇浓度

Concentration Detection of Ethanol in Huai Shi Ye by Headspace GC

目的:建立气相色潽法测定怀氏液中乙醇的含量。方法:采用安捷伦DB-ALC1型气相色谱柱(30 m×0.53 mm,3.0μm),氢火焰离子化检测器(FID)温度:250℃,柱温:80℃,进样口温度:200℃,N_2流速:5ml·min^(-1),辅助加热区:115℃。以叔丁醇为内标进行定量采用顶空进样1.0μl。结果:被测物均能得到良好的分离,乙醇在3.1770~15.8850 mg范围内峰面积线性关系良好(r=0.999 6);平均回收率为98.7%,RSD为2.84%(n=6);高、中、低浓度的精密度均小于2.5%。结论:本方法灵敏快速,准确可靠,可用于怀氏液中乙醇浓度测定。

Objective： To establish a detection method for ethanol in HuaiShi province Ye. Methods： Ethanol in HuaiShi Ye was determined by GC with a DB-ALC1 capillary column（30 m ×0.53 mm,3.0 μm）, an FID detector and nitrogen as the cartier gas. The injector temperature was 200℃C, the detector temperature was 250℃ and the column temperature was 80℃. The flow rate of N2 was 5 ml ~ min-1. Tertbutyl alcohol was used as the internal standard, and 1.0p,1 sample was injected by a headspace injector. Results： The solvents were completely separated, and the calibration curve of ethanol had good linear relationship within the range of 3. 177 0- 15. 885 0 mg（ r =0.999 6） , and the recovery was 98.7%. The intra-day precision of different concentrations was less than 2.5%. Condusion： The method is sensitive, rapid, accurate and reliable, and can be used for the determination of ethanol in HuaiShi Ye.