哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义

Roles of GITR/GITRL signal system expression at bloodstream lymphocytes in children with asthma

目的：观察 GITR 及其配体（GITRL）在哮喘患儿外周血淋巴细胞的表达，探讨哮喘的部分炎症机制。方法50例儿童重度哮喘患儿（哮喘组）分别在急性期和缓解期取静脉血，流式细胞术检测CD ＋4 CD25＋Treg 细胞 GITR 和 CD ＋4 T 细胞 GITRL 的平均荧光强度，与32例健康儿童作对照，进行统计分析。结果哮喘组急性期 CD ＋4 CD25＋Treg 细胞 GITR 的表达水平为（24．2±8．2）MFI，显著低于对照组的（28．5±6．0）MFI（t ＝2．5，P ＜0．05）。哮喘组急性期与对照组 CD ＋4 T 细胞 GITRL 的表达水平分别为（5．2±3．6）MFI与（4．6±1．0）MFI，差异无统计学意义（t ＝1．1，P ＞0．05）。哮喘组 CD ＋4 CD25＋Treg 细胞 GITR 的表达水平在缓解期[（29．5±8．3）MFI]显著高于急性期（t ＝－9．9，P ＜0．01）；CD ＋4 T 细胞 GITRL 的表达水平在哮喘组急性期、缓解期（5．7±3．6）MFI 之间差异无统计学意义（t ＝－1．6，P ＞0．05）。CD ＋4 CD25＋Treg GITR 与 CD ＋4 T GITRL 的表达之间无显著相关性（n ＝132，r ＝0．04，P ＞0．05）。结论哮喘急性期患儿 CD ＋4 CD25＋Treg 细胞GITR 表达下降，在缓解期得到回升，而 CD ＋4 T 细胞 GITRL 的表达水平没有变化，GITR／GITRL 信号系统可能参与了哮喘的炎症过程。

Objective To investigate the expression of blood CD ＋4 CD ＋25 Treg GITR,CD ＋4 T cell GITRL in children with asthma,and the role of them in asthmatic inflammation.Methods 50 cases of severe asthma were selected,and were controlled with thirty two healthy children.The venous blood was collected both in the period of acute episode and clinic remission.The mean fluorescence intensity of CD ＋4 CD ＋25 Treg GITR and CD ＋4 T cell GITRL was detected by flow cytometry.Results The expression of CD ＋4 CD ＋25 Treg GITR in the asthma acute period group was （24.2 ±8.2）MFI,which was significantly lower than （28.5 ±6.0）MFI in the control group（t =2.5,P〈0.05）.While there was no significant difference of CD ＋4 T cell GITRL expression between the asthma acute period group[（5.2 ±3.6）MFI]and the control group[（4.6 ±1.0）MFI]（t =1.1,P〉0.05）.Moreover,the expression of CD ＋4 CD ＋25 Treg GITR in the asthma in remission group after treatment was （29.5 ±8.3）MFI,which was significantly higher than that in acute period group before treatment（t =-9.9,P〈0.01 ）.Whereas,there was no significant difference of CD ＋4 T cell GITRL expression in asthma group between in remission after treatment[（5.7 ±3.6）MFI] and in acute period before treatment（t =-1.6,P 〉0.05）.Furthermore,there was no significant correlation between levels of CD ＋4 CD ＋25 Treg GITR and CD ＋4 T cell GITRL.Conclusion The level of CD ＋4 CD ＋25 Treg GITR in acute period asthmatic patients was decreased,but it was increased in remission,but no changes of CD ＋4 T cell GITRL expression were observed.GITR/GITRL signal system might be involved in the asthmatic inflammation procession.