改良羊血巧克力培养基的应用与评价

Application and evaluation of modified sheep blood chocolate culture medium

目的评价改良羊血巧克力培养基的质量与应用价值。方法将ATCC10211流感嗜血杆菌接种到改良前后两种培养基上，比较两种培养基上嗜血杆菌的平均生长指数（GI值）；对352份经筛选的合格痰标本进行流感嗜血杆菌检测，比较两种培养基的阳性分离率。结果传统羊血巧克力培养基GI值为（3．69±0．58），改良羊血巧克力培养基GI值为（15．08±1．34），两种培养基GI值差异有统计学意义（t=25．31，P〈0．01）。352份痰标本在改良羊血巧克力培养基上流感嗜血杆菌检出41例，阳性检出率11．65％。352份痰标本在传统羊血巧克力培养基上流感嗜血杆菌检出18例，阳性检出率5．54％。流感嗜血杆菌在两种巧克力培养基上的分离率差异有统计学意义（X2=21．04，P〈0．05）。结论流感嗜血杆菌在改良羊血巧克力培养基上生长良好，菌落明显，极易识别，有助于临床标本中流感嗜血杆菌的检出。

Objective To evaluate the quality and application value of improved sheep blood chocolate medium. Methods The ATCC 10211 of Haemophilus influenzae was inoculated into the modified medium and unmodified medium, the average growth index （GI） of Haemophilus influenzae in two kinds of culture medium was compared. Based on 352 selected qualified sputum specimens for detection of Haemophilus influenzae, the positive isolation rate of medium was compared between the two groups. Results GI value of the traditional blood chocolate culture medium was （3.69±0.58） ,which was significantly lower than （ 15.08±1.34） of the improved sheep blood chocolate culture medium, the difference was significant （ t = 25.31, P 〈0. 01 ）. 352 sputum specimens in the improved sheep blood chocolate culture medium, Haemophilus influenzae detected in 41 cases, the positive rate was 11.65 %. And 352 sputum specimens in traditional sheep blood chocolate culture, Haemophilus influenzae detected in 18 cases, the positive rate was 5.54%. There was significant differenceof Haemophilus influenzae in separation based on 2 kinds of chocolate culture medium （ X2 = 21.04, P 〈 0.05 ）. Conclusion Haemophilus influenzae significantly improved sheep blood colony in chocolate culture medium, grows well, easily to be identified, which helps to detect sputum specimens of Haemophilus influenzae.