基因芯片法和培养法在细菌性痢疾中的应用对比

Application comparison of gene chip and culture in bacterial dysentery and the its comparative study

目的比较基因芯片法和培养法在细菌性痢疾诊断中的临床价值。方法采集临床诊断为细菌性痢疾的患者脓血黏液便,应用基因芯片技术进行病原菌的检测,并将结果与传统的培养法进行比较。结果 108例患者中,基因芯片法检测出痢疾杆菌37例,沙门菌13例,大肠埃希菌2例,病原菌检出率为48.1%;培养法检测出痢疾杆菌24例,沙门菌5例,病原菌检出率为26.9%;基因芯片法和培养法对病原菌、痢疾杆菌、沙门菌的检出率差异均有统计学意义(P<0.05)。培养法患者的基因芯片法均为阳性。培养法平均检测时间为48 h^72 h,基因芯片法平均检测时间为4.5 h。结论基因芯片技术对细菌性痢疾病原菌的检出具有快速、敏感的特点,可替代传统的培养法,在细菌性痢疾的早期诊断和治疗中具有重要的临床价值。

Objective To compare the clinical value of gene chip and the cultivation in the diagnosis of bacterial dysentery.Methods Collecting clinical diagnosed bacillary dysentery in patients with purulent mucus,and to detect the pathogenic bacteria with gene chip technology,and the results were compared with the traditional cultivation method. Results In 108 patients,with the gene chip method,37 cases of Shigella,13 cases of Salmonella and 2 cases of Escherichia coli were detected,and the detection rate of pathogen was 48. 1%; with cultivation method,24 cases of Shigella and 5 cases of Salmonella were detected,and the detection rate of pathogen was 26. 9%; the differences on detection rates of pathogenic bacteria,Shigella,and Salmonella in gene chip method and the cultivation method all had statistical significance（ P〈0. 05）. And genes were all positive with both gene chip and cultivation. The average detection time with cultivation was 48 h-72 h,and that with gene chip was4. 5 h. Conclusion Gene chip technique is rapid and sensitive for the detection of pathogenic bacteria of bacterial dysentery,which can replace the traditional cultivation method,and has important clinical value in early diagnosis and treatment of bacillary dysentery.