儿童9种呼吸道病原体的IgM抗体检测结果分析

A clinical analysis of 9 pathogenic IgM antibodies in children with respiratory tract infection

目的回顾性分析南京儿童医院呼吸系统患者9种呼吸道病原体感染的临床分布情况,有助于临床医生对儿童呼吸道感染疾病的诊治。方法采用间接免疫荧光法对血清中9种病原体的Ig M抗体进行检测,并对其感染病原体情况进行回顾性分析。结果入选患儿共12 042例,感染阳性4 622例,阳性率为38.38%;总体阳性项目数5 509项,总检出率为45.75%。阳性率高的前5种病原体分别为MP(34.16%)、RSV(5.95%)、ADV(2.8%)、PIV(1.43%)、Flu B(0.73%)。MP、Flu B和PIV的阳性率女童高于男童,差异有统计学意义(P<0.05)。除COX病原体外,其他病原体的感染在不同年龄组的分布差异均具有统计学意义(P<0.05)。大部分病原体的感染呈季节性分布且差异具有统计学意义(P<0.05)。结论 MP、RSV和ADV是儿童呼吸道感染的主要病原体,MP、FluB和PIV感染阳性率女童高于男童,并且大多病原体感染均具有年龄和季节分布的特点。

Objective To retrospectively investigate the clinical distribution of 9 respiratory pathogens in children with respiratory tract infection in Nanjing Children＇s Hospital,so as to help the clinical treatment of respiratory tract infection in children.Methods The Ig M antibodies of 9 pathogens in the serum were detected using indirect immunofluorescence assay. Then a retrospective analysis was performed with those data. Results Totally 12 042 cases were selected,in which 4 622 cases（ 38. 38%） were positive. The quantity of positive items was 5 509 with the total detection rate of 45. 75%. The top five positive rates of each pathogen were as follows： MP（ 34. 16%）,RSV（ 5. 95%）,ADV（ 2. 8%）,PIV（ 1. 43%）,Flu B（ 0. 73%）.Positive rates of MP,Flu B and PIV of girls were higher than those of boys,and the difference was statistically significant（ P〈0. 05）. Except for COX infection,the distribution differences of other pathogens infection were statistically significant in different age groups（ P〈0. 05）. In addition,infection of most them displayed a seasonal distribution,and the difference was statistically significant（ P〈0. 05）. Conclusion MP,RSV and ADV are the main pathogens of respiratory infection in children; and the infection rates of MP,Flu B and PIV among boys were more higher than that among girls with a seasonal distribution.