Nogo-B在子痫前期胎盘和正常胎盘组织中的差异表达及其与病情的相关性分析被引量：3

Differential expression of Nogo-B in preeclampsia placental tissue and normal placental tissue and its correlation with illness-related molecule expression

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摘要目的:研究Nogo-B在子痫前期胎盘组织和正常胎盘组织中的差异表达及其与病情相关分子表达的相关性。方法:收集子痫前期产妇和正常妊娠产妇的胎盘组织,采用PCR方法检测Nogo-B和凋亡基因(Fas、Caspase-3、Caspase-9)的mRNA含量,采用Elisa方法检测炎症因子(TNF-α、IL-1β、IL-6、IL-8、CD40L、VCAM-1)和内皮损伤分子(LOX-1、ox-LDL、PTX3、ADM)的蛋白含量。结果:子痫前期胎盘组织中Nogo-B的mRNA含量显著高于正常胎盘组织(P<0.05)且病情越严重、Nogo-B的mRNA含量越高;子痫前期胎盘组织中Fas、Caspase-3、Caspase-9的mRNA含量以及TNF-α、IL-1β、IL-6、IL-8、CD40L、VCAM-1、LOX-1、ox-LDL、PTX3、ADM的蛋白显著高于正常胎盘组织(P<0.05);Nogo-B的mRNA含量与Fas、Caspase-3、Caspase-9的mRNA含量以及TNF-α、IL-1β、IL-6、IL-8、CD40L、VCAM-1、LOX-1、ox-LDL、PTX3、ADM的蛋白呈正相关。结论:Nogo-B在子痫前期胎盘组织中的表达量显著升高,该分子能够通过调节凋亡基因、炎症因子和内皮损伤分子的生成来参与子痫前期的发生。 Objective： To study the differential expression of Nogo-B in preeclampsia placental tissue and normal placen- tal tissue and its correlation with illness--related molecule expression. Methods.. Placental tissue of preeclampsia puerperas and normal pregnancy puerperas was collected. PCR method was used to detect mRNA contents of Nogo-B and apoptosis genes （Fas, Caspase-3 and Caspase-9） and ELISA was used to detect protein contents of inflammatory factors （TNF-α, IL-1β, IL 6, IL-8, CD40L and VCAM-1） and endothelial injury molecules （LOX-1, ox-LDL, PTX3 and ADM）. Results： mRNA content of Nogo-B in preeclampsia placental tissue was significantly higher than that in normal placental tissue. The more severe the dis- ease, the higher the mRNA content of Nogo-B. mRNA contents of Fas, Caspase-3 and Caspase-9 as well as protein contents of TNF-α, IL-1β, IL-6, IL-8, CD40L, VCAM-1, LOX-1, ox-LDL, PTX3 and ADM in preeclampsia placental tissue were signif- icantly higher than those in normal placental tissue; mRNA content of Nogo-B was positively correlated with mRNA contents of Fas, Caspase-3 and Caspase-9 as well as protein contents of TNF-α, IL-1β, IL-6, IL-8, CD40L, VCAM-1, LOX-1, ox- LDL, PTX3 and ADM. Gonclusion： Nogo-B expression in preeclampsia placental tissue significantly increases, and the mole-cule can regulate the generation of apoptosis genes, inflammatory factors and endothelial injury molecules to he involved in the occurrence of preeclampsia.

作者许芙蓉王海燕

机构地区武汉市十一医院妇产科

出处《海南医学院学报》 CAS 2016年第1期16-19,共4页 Journal of Hainan Medical University

基金武汉市卫计委公共卫生科研项目(WG13E85)~~

关键词子痫前期 Nogo—B 内皮损伤分子凋亡基因炎症因子 Preeclampsia Nogo-B Endothelial injury Apoptosis Inflammatory factors

分类号 R714.2 [医药卫生—妇产科学]

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