短程EOF方案区域动脉灌注化疗与常规化疗用于进展期胃癌的有效性和安全性评估

Efficacy and safety assessment of short EOF program regional arterial infusion chemotherapy and conventional chemotherapy for advanced gastric cancer

目的:研究短程EOF方案区域动脉灌注化疗与常规化疗用于进展期胃癌的有效性和安全性。方法:选择在本院确诊为进展期胃癌的66例患者进行研究,术前进行短程EOF方案化疗并随机分为两组,观察组接受短程EOF方案区域动脉灌注化疗,对照组接受短程EOF方案静脉化疗。检测两组肿瘤组织中的凋亡细胞的数目及凋亡基因的含量、血清中肝肾功能指标以及cfDNA甲基化程度。结果:(1)有效性指标:观察组胃癌组织中凋亡细胞的数目多于对照组,Caspase-3、Caspase-9、Fas、FasL的mRNA含量高于对照组,血清中p16、RNF180、SFRP2、SOX17、RUNX甲基化比例低于对照组,差异具有统计学意义(P<0.05);(2)安全性指标:观察组血清RBP、CysC、ALT、AST含量低于对照组,差异具有统计学意义(P<0.05)。结论:短程EOF方案区域动脉灌注化疗能够更为有效的杀灭癌细胞、降低肿瘤相关基因的甲基化程度、减轻肝功能和肾功能损伤,其有效性和安全性均优于常规化疗。

Objective： To investigate the efficacy and safety of short EOF program regional arterial infusion chemothera- py and conventional chemotherapy for advanced gastric cancer. Methods.. A total of 66 patients diagnosed as advanced gastric cancer in our hospital were enrolled for the study. Before undergoing operation, they were given short EOF program chemo- therapy and were randomly divided into two groups. Observation group received short EOF program regional arterial infusion chemotherapy and control group received short EOF program intravenous chemotherapy. Then number of apoptosis cells and contents of apoptosis genes in the tumor tissue, serum liver and kidney function indicators as well as efDNA methylation degree of two groups were detected and compared. Results= （1） Indicators of efficacy： the number of apoptosis cells in gastric cancer tissue of observation group was more than that of control group, mRNA levels of Caspase-3, Caspase-9, Fas and FasL were higher than those of control group, and serum p16, RNF180, SFRP2, SOX17 and RUNX methylation ratios were lower than those of control group; （2） Indicators of safety： serum RBP, CysC, ALT and AST contents of observation group were lower than those of control group. Conclusion： Short EOF program regional arterial infusion chemotherapy can more effectively kill cancer cells, reduce methylation degree of tumor-associated genes and decrease liver function and kidney function damage; both efficacy and sa{ety of this therapy are better than that of the conventional chemotherapy.