子痫前期胎盘中Gadd45α的表达及敲低Gadd45α对滋养细胞HTR8/Svneo的调节作用被引量：5

Gadd45α expression in preeclampsia placenta and effect of Gadd45α on trophoblast HTR8/Svneo

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摘要目的:研究子痫前期胎盘中Gadd45α的表达量及敲低Gadd45α对滋养细胞HTR8/Svneo的调节作用。方法:收集子痫前期胎盘组织和正常胎盘组织,分别采用荧光定量PCR法和免疫印迹法检测Gadd45α的mRNA含量和蛋白含量;培养滋养细胞HTR8/Svneo,转染Gadd45α的siRNA后检测细胞的侵袭能力及侵袭相关分子的表达量。结果:子痫前期胎盘组织中Gadd45α的mRNA含量和蛋白含量高于对照组;转染Gadd45α的siRNA后,HTR8/Svneo细胞中Gadd45α的mRNA含量和蛋白含量均明显降低,侵袭细胞的数目以及MMP1、MMP2、MMP3、MMP9的表达量均明显增加。结论:子痫前期胎盘中Gadd45α的表达量异常升高;抑制滋养细胞HTR8/Svneo中Gadd45α的表达量能够促进的侵袭、增加MMPs分子的表达。 Objective： To study the Gadd45α expression in preeclampsia placenta and the regulation effect of Gadd45α on trophoblastHTR8/Svneo. Method： Preeclampsia placenta tissue and normal placental tissue were collected, mRNA content and protein content of Gadd45α were detected by real--time PCR and Western blotting. Trophoblast HTRS/Svneo were cultured and cell invasion ability and invasionrelated molecules were detected after transfection of Gadd45α siRNA. Results.. mRNA con- tent and protein content of Gadd45α in preeclampsia placenta mRNA content and protein content was higher than normal pla- cental tissue. After transfection of Gadd45αsiRNA, mRNA content and protein content of Gadd45α in HTR8 / Svneo cell were significantly reduced, the number of invasive cells and expression levels of MMP1, MMP2, MMP3, MMP9 were significantly increased. Conclusion： The expression of Gadd45α in preeclampsia placenta is abnormally increased. Suppressing expression of Gadd45α in trophoblast cells HTR8 / Svneo can promote cell invasion and increaseexpression of MMPs molecules.

作者王丽赵琳

机构地区辽宁省盘锦市中心医院妇产分院

出处《海南医学院学报》 CAS 2016年第2期119-121,125,共4页 Journal of Hainan Medical University

基金辽宁省科技厅博士启动项目(No.20111107)~~

关键词子痫前期生长阻滞和DNA损伤45α 基因敲低基质金属蛋白酶 Preeclampsia Growth arrest and DNA damage 45α Gene knockdown Matrix metalloproteinase

分类号 R714.2 [医药卫生—妇产科学]

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