极低频电磁场对骨肉瘤HOS细胞的体外生物效应

Preliminary biological effects of extremely low frequency electromagnetic field on the osteosarcoma HOS cells in vitro

目的:观察极低频电磁场(extremely low frequency electromagnetic field,ELF-EMF)对骨肉瘤HOS细胞的增殖、凋亡、克隆形成及侵袭能力的作用,筛选出对骨肉瘤HOS细胞抑制作用最佳的极低频电磁场参数。方法:体外培养骨肉瘤HOS细胞,分别固定电磁场频率为1Hz、5Hz、10Hz、15Hz、20Hz、30Hz、40Hz、50Hz,选择不同电磁场强度0.1mT、1mT、5mT、10mT、15mT,分组进行辐照,并设立对照组。采用CCK-8法检测细胞增殖情况并计算抑制率。经统计学分析后,筛选出2组对HOS细胞抑制作用较强的ELF-EMF参数,再通过检测其细胞凋亡、克隆形成及侵袭能力进行验证。结果:各组OD值经统计学分析,显示(1Hz,0.1mT)实验组细胞增殖抑制率最高,(40Hz,10mT)实验组次之(P<0.05)。流式细胞术结果表明,这2组的HOS细胞凋亡比率均高于对照组(P<0.05)。细胞克隆实验中,对照组克隆率最大,(1Hz,0.1mT)实验组次之,而(40Hz,10mT)实验组克隆率最小(P<0.05)。侵袭实验中,(40Hz,10mT)实验组穿过小室基底膜的细胞数量明显低于其他两组(P<0.05)。结论:(40Hz,10mT)组ELF-EMF参数对骨肉瘤HOS细胞有更明显抑制增殖作用,促使细胞凋亡,降低细胞的克隆能力,并减低其侵袭能力。

Objective：By the ELF - EMFg （extremely low frequency electromagnetic field） effects on the osteosarcoma HOS cells＇proliferation, apoptosis, invasion and colony formation, the optimal inhibition parameter of ELF - EMF on osteosarcoma HOS cells could be filtered finely. Methods ： HOS cells were irradiated with different magnetic field intensities（0.1 mT, 1 mT, 5mT, 10mT, 15mT） , which were fixed with magnetic field frequencies （ 1Hz, 5Hz, 10Hz, 15 Hz,20Hz,3OHz ,40Hz, 50Hz ） , comparing with the control groups, in vitro. HOS cells＇ proliferation and inhibition rate was calculated with CCK -8 assay. With the statistical analysis, we selected two stronger inhibition of ELF - EMF parameter on HOS cells, and validated them with the assay of apoptosis, colony formation and invasion again. Results ： When the OD values of every group were analyzed,the cell inhibition rate of （1Hz ,0.1 mT） group of ELF -EMF irra- diation was the most,and （40Hz, 10mT） group was the second （P 〈 0.05 ）. Both of the two groups＇apoptosis rates were higher than the control group,with the flow cytometry （P 〈 0.05 ）. In the colony formation assay, the most effi- ciency of clone formation was the control group,the second was （1Hz,0. hnT） group,and the least was （40Hz, 10mT） group（P 〈 0.05）. In addition,the number of invased HOS cells in （40Hz, 10mT） group was obviously less than others （ P 〈 0.05 ）. Conclusion： （ 40 Hz, 10mT） group of the ELF - EMF parameter can significantly inhibite the proliferation of osteosarcoma HOS cells, which can also induce apoptosis, reduce the ability to clone and slow down the invasion.