联氨基姜黄素脂质体纳米颗粒对乳腺癌细胞增殖、凋亡、侵袭和迁移的影响

Curcumin affects- hydrazino liposomal nanoparticles on breast cancer cell proliferation,apoptosis,invasion and migration

目的:研究联氨基姜黄素脂质体纳米颗粒(HC-NPs)对乳腺癌细胞的抑制作用及其机制。方法:制备HC-NPs,培养人乳腺癌MDA-MB-231细胞。以对数生长期的细胞为研究对象。分成对照组和实验组,对照组及实验组分别予以脂质体纳米颗粒空载(NPs)、HC-NPs处理。测定细胞生长抑制率、移行愈合率,并测定相关蛋白Cyclin D1、Bcl-2、Bax、Survivin、MMP-9、p-STAT3的表达情况。结果:HC-NPs处理人乳腺癌MDA-MB-231后,与NPs对照组相比,可明显抑制细胞的增殖,差异有统计学意义(P<0.05)。FCM法检测实验组凋亡率高于对照组,差异有统计学意义(P<0.05)。细胞周期测定有显著差异,P均<0.05。HC-NPs作用于人乳腺癌MDA-MB-231细胞24h后,细胞周期停滞在G_2期。Transwell法检测到HC-NPs处理后,测定侵袭迁移,与对照组比较,HC-NPs组侵袭迁移能力明显低于对照组(P均<0.05)。Western blotting法测定相关蛋白p-STAT3、Cyclin D1、Bcl-2、Survivin、MMP-9的表达情况,HC-NPs组明显降低且低于对照组,而Bax明显增高且高于对照组,总STAT3水平无明显差异。结论:HC-NPs对乳腺癌细胞的抑制作用机制与下调JAK/STAT信号通路有关。

Objective： To study the effect of linking amino liposomal curcumin nanoparticles （ HC - NPs） on breast cancer cells and its mechanism. Methods： Prepared the HC - NPs, cultured human breast cancer MDA - MB - 231 cells in logarithmic growth phase cells for the study, and cells were divided into control group and the experimental group. The control group was not with special treatment, the experimental group were given NPs. HC - NPs processing and measuring the rate of cell growth inhibition, transitional healing rate and determine associated protein CyclinD1, Bcl - 2, the expression of Bax, Survivin, MMP - 9, p - STAT3. Results ： HC - NPs treated human breast cancer MDA -MB -231, compared with liposomal nanopartieles load （NPs） in the control group, significantly inhibited cell pro- liferation in the experimental group than the control group（ P 〈 0.05 ）. Apoptosis rate was higher in the experimental group（ P 〈0.05 ）. There were significant differences in cell cycle measurement（ P 〈0.05 ）. HC -NPs role in human breast cancer MDA - MB -231 cells after 24h, cell cycle arrest in G2. Invasion and migration compared with the con- trol group （ P 〈 O. 05 ）. HC - NPs invasion and migration group was significantly lower than the control group. MMP - 9 was significantly reduced and relatively lower than the control group,while Bax increased significantly higher. Conclusion ：The effect of HC - NPs on breast cancer cells was related with down JAK/ STAT signaling pathway.