摘要
目的研究神经节苷脂(GM1)对急性脊髓损伤大鼠Bcl-2表达的变化。方法选取72只成年健康SD大鼠,按Nystrom法建立大鼠脊髓损伤模型,按随机数字表法分为假手术组24只、脊髓损伤组24只、GM1组24只,脊髓损伤组和GM1治疗组依据脊髓损伤后的不同时间点再细分为1d、7d和14d三个亚组。伤后第1天、第7天和第14天分别用BBB评分和斜板试验观察大鼠运动功能的恢复情况。免疫组化方法和Western blot方法检测三组大鼠脊髓损伤部位Bcl-2的表达。结果术后7d和14d,GM1治疗组BBB评分和斜板试验明显优于脊髓损伤组(<0.05)。假手术组大鼠脊髓有大量Bcl-2阳性细胞,在脊髓损伤后1d时,损伤部位Bcl-2阳性细胞数及表达水平明显减少,7 d时略有上升,14d时仍明显低于假手术组(<0.05),与相同时间点的SCI组比较,GM1组大鼠脊髓损伤部位的Bcl-2阳性细胞数及表达水平明显上升(<0.05)。结论上调Bcl-2蛋白的表达可能是GM1治疗急性脊髓损伤的机制之一。
Objective To study the effect of monosialoganglioside（GM1） on the expression of Bcl-2 in acute spinal cord injury of SD rat. Methods 72 SD healthy adult rats were randomly divided into the sham group（24）, the spinal cord injury group（24）, GM1 group（24） at different time points（1d, 7d,14d）. The spinal cord injury（SCI） was induced with Nystrom＇s way. The recovery of motor function after SCI was assessed with Basso-Beattie-Bresnahan（BBB）scale and tilt board test the 1d, 7d and 14 d after SCI. The expression of Bcl-2 was observed by immunochemistry and western blot methods. Results BBB scores and tilt board test were better in GM1 group than in SCI group（P〈0.05）the 7d and 14 d after SCI. The number of Bcl-2 positive cells and expression level of Bcl-2in spinal cord were decreased obviously in SCI group than in sham group（P〈0.05）, but significantly higher in GM1 group than in SCI group（P〈0.05）. Conclusion The therapeutic effect of GM1 on the acute spinal cord injury might be related to the upregulation of the expression of Bcl-2.
出处
《解剖科学进展》
2015年第6期632-634,共3页
Progress of Anatomical Sciences
基金
辽宁省教育厅科研基金(L2010687)
