宁泌泰胶囊的抗炎作用研究

Anti-inflammatory effect of Ningmitai Capsules

目的探讨宁泌泰胶囊的抗炎作用及其作用机制。方法采用1%角叉菜胶致小鼠足趾肿胀、2%巴豆油丙酮溶液致小鼠耳肿胀模型,观察宁泌泰胶囊(0.5、1.5、4.5 g/kg)对小鼠肿胀的抑制作用。考察25、50、100μg/m L宁泌泰胶囊加入RAW264.7细胞作用48、72 h后的细胞毒性。运用双荧光素酶报告基因系统检测宁泌泰胶囊对NF-κB转录活性的影响。结果与模型组比较,宁泌泰胶囊(4.5 g/kg)对角叉菜胶致小鼠足肿胀、巴豆油致小鼠耳肿胀具有明显的抑制作用(P<0.05)。宁泌泰胶囊对RAW264.7细胞没有细胞毒性。50、100μg/m L宁泌泰胶囊内容物对NF-κB的转录激活具有明显的抑制作用(P<0.01)。结论宁泌泰胶囊具有抗炎作用,其作用机制可能是通过NF-κB通路来抑制炎症过程。

Objective To study the anti-inflammatory effect of Ningmitai Capsules and its mechanism.Methods The models of 1% carrageenan-induced mice paw edema and 2% oleum crotonis-induced ear edema were established, and the inhibition of swelling in mice adminisered with Ningmitai Capsules were observed. Cytotoxicity of 25, 50, and 100 μg/mL Ningmitai Capsules on RAW264.7 cells were studied after 48 and 72 h. Dual luciferase report gene system was used to detect the transcriptional activity of NF-κB. Results Compared with model group, Ningmitai Capsules with the dosage of 4.5 g/kg could obviously inhibit the mice foot swelling induced by carrageenan and the ear edema induced by oleum crotonis （P〈0.05）. There was no cytotoxicity of Ningmitai Capsules on RAW264.7 cells. Ningmitai Capsules with the concentration of 50 and 100 μg/mL could inhibit the transcriptional activity of NF-κB （P〈0.01）.Conclusion Ningmitai Capsules have anti-inflammatory effect, which is related to inhibition of inflammatory processes through NF-κB pathway.