乙醇回流法提取甜菜树总黄酮及其抗氧化性评价

Extraction of Total Flavonoids from Yunnanopilia longistaminea by Ethanol Reflux Method and Evaluation of Its Antioxidant Activity

以甜菜树为原料,总黄酮提取率为考察指标,在单因素试验的基础上,通过正交试验优化乙醇回流法提取总黄酮的最佳工艺条件,并就总黄酮对1,1-二苯基-2-苦基肼自由基(DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O2-·)的清除作用进行了初步研究。结果表明,甜菜树总黄酮最佳提取工艺参数为:乙醇体积分数85%,料液比1∶40(g/m L),提取温度80℃,提取时间180 min,在该提取工艺条件下,得到甜菜树总黄酮平均提取率为1.67%。总黄酮质量浓度在0.009 3~0.046 5 mg/m L时,对DPPH·、·OH、O2-·的清除率可分别达到88.39%、91.47%、46.29%,说明甜菜树总黄酮具有较强的体外抗氧化活性。

In order to increase the extraction rate of total flavonoids, the ethanol reflux extraction conditions of total flavonoids from Yunnanopilia longistaminea was optimized by L9(34) orthogonal test based on the single-factor tests.Meanwhile, the antioxidant effects of obtained total flavonoids on 1,1- phenyl-2- base of the radical(DPPH·), hydroxyl radical(·OH) and superoxide anion radical(O2-·) were studied. The results showed that, the optimum extraction parameters were: the volume fraction of ethanol as extractant was 85%, ratio of solid to liquid was 1∶40(g/m L),extraction temperature was 80 ℃, and extraction time was 180 min. Under the extraction conditions, the average extraction rate of total flavonoids from Yunnanopilia longistaminea was 1.67%. When the total flavonoids concentration was in the range of 0.009 3 to 0.046 5 mg/m L, the clearance rate of total flavonoids on DPPH·, ·OH and O2-· were88.39%, 91.47% and 46.29%, respectively, implying such material had a strong antioxidant activity in vitro.