高效液相色谱法测定保健品中白藜芦醇的含量

Determination of resveratrol in health products by HPLC

目的应用高效液相色谱法测定保健品中白藜芦醇的含量。方法采用Alltima C18色谱柱（150mm×4.6 mm,5μm）,以乙腈-0.05%磷酸（30：70,v/v）为流动相,流速1.0 mL·min^-1,检测波长306nm。结果白藜芦醇在10.04-251.0 mg·L^-1与峰面积线性关系良好,线性相关系数（r）为0.9999;专属性强,白藜芦醇和样品中其他成分以及强制降解产物之间有良好的分离度;精密度和稳定性良好,RSD分别为0.12%和0.16%;平均加样回收率为99.8%。结论该方法准确、简单、快速,可作为保健品中白藜芦醇的含量测定方法。

Objective To establish an HPLC method for the determination of resveratrol in health products. MethodsAn Alltima C18 column（150 mm×4.6 mm, 5 μm） was used, with acetonitrile-0.05% phosphoric acid（30 ：70, v/v） as the mobile phase at the flow rate of 1.0 mL·min^- 1. The detection wavelength was 306 nm. Results The method showed good linearity at 10.04- 251.0 mg·L^-1 with a correlation coefficient of 0.9999; the study showed satisfactory resolutions within resveratrol, other ingredients and forced degradation products; the precision and stability were satisfactory with the RSD of 0.12% and 0.16%, respectively. The average recovery was 99.8%. Conclusion The established method is fast, simple and accurate, and can be used for the determination of resveratrol in health product.