免疫组织化学染色法在特发性血小板减少性紫癜患者幽门螺杆菌感染检测中的应用

Application of immunohistochemical staining in detection of Helicobacter pylori infection in patients with idiopathic thrombocytopenic purpura

目的评价免疫组织化学染色法对检测特发性血小板减少性紫癜（ITP）患者幽门螺杆菌（Hp）感染的临床价值。方法对就诊的79例ITP患者行胃内窥镜检查（检查前行尿素·4C呼气试验），并取胃黏膜进行活组织检查，行苏木精一伊红（HE）染色及Hp免疫组织化学染色。结果尿素14C呼气试验及Hp免疫组织化学染色检测ITP患者Hp感染的阳性率分别为51．9％（41／79）、67．1％（53／79），两者差异具有统计学意义（χ2=71．53，P=0．023）。结论免疫组织化学染色法检测Hp直观清楚，结果准确可靠，是检测ITP患者Hp感染的有效方法。

Objective To analyze the clinical value of immunohistochemical staining of Helicobacter pylori （Hp） in patients with idiopathic thrombocytopeuic purpura （ITP）. Methods A total of 79 ITP patients were performed gastroscopy and biopsy in gastric mucosa. Hematoxylin and eosin （HE） staining and immunohistochemical staining were performed. Before gastroscopy, 14C-urea breath test was used to detect the Hp infection. Results The positive rates of Hp infection detected by 14C-urea breath test and Hp immunohistochemieal staining in 79 cases of ITP were 51.9 % （41/79） and 67.1% （53/79） respectively, the difference was statistically significant （χ2 = 71.53, P = 0.023）. Conclusions Hp immunohistochemistry staining is clear and accurate,and easy to observe. It is an effective method for detection of Hp infection in ITP patients.