摘要
目的:观察去卵巢骨质疏松症(OP)大鼠模型骨及肌肉组织Notch信号蛋白活性变化,探究补肾中药防治OP的分子生物学机制。方法:75只大鼠随机分为5组:正常组、假手术组、模型组、补肾中药组、福善美组,以去卵巢法建立绝经后OP大鼠模型,补肾中药组运用补肾中药干预8周后以双能X线骨密度分析仪检测骨密度,ELISA法检测血清OPG、RANKL,骨及肌组织HES1、JAG1以及Notch1蛋白表达。结果:与正常组比较,模型组骨密度及血清OPG明显减少(P<0.01),血清RANKL明显升高(P<0.01),骨及肌肉组织Notch信号蛋白表达明显减少(P<0.01);与模型组相比较,各治疗组骨密度、血清OPG及骨组织Noth信息蛋白均有不同程度的升高(P<0.05,P<0.01)。结论:OP的发生机制与Notch信号通路有关;补肾中药通过激活Notch信号传导通路,促成骨分化,抑制破骨活性,起到防治OP的作用。
Objective: To explore the molecular biological mechanism of the kidney-reinforcing herbs in preventing and treating osteoporosis by observing the activity changes of Notch signaling protein in bone and muscular tissues of the ovariectomized model rats with osteoporosis. Methods: Seventy-five rats were randomly divided into five group: normal group, sham operation group, model group, Fosamax group and kidney-tonifying Chinese herbs group, 15 in each group. Rats in kidneytonifying Chinese herbs group were intervened with kidney-reinforcing herbs for 8 weeks. Bone mineral density was determined by dual-energy X ray bone densitometer, and serum OPG, RANKL, bone and muscular tissue HES1, JAG1 and the protein expression of Notch1 were determined by ELISA method. Results: Compared with normal group, the bone mineral density and serum OPG were significantly decreased in model group(P<0.01), while serum RANKL was significantly increased(P<0.01), the Notch signal protein expression of bone and muscular tissues in model group was significantly decreased(P<0.01). The bone mineral density in each treatment group was increased with varying levels compared with model group(P<0.05, P<0.01). Conclusion: The mechanism of osteoporosis is related to Notch signal pathway. Kidney-reinforcing Chinese herbs has the effects of preventing osteoporosis by activating the Notch signal pathway, promoting the differentiation of osteoblast and suppressing the activity of osteoclast.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2016年第8期3208-3212,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家重点基础研究发展计划(973计划)项目(No.2010CB530400)
国家自然科学基金项目(No.81273612)~~
关键词
骨质疏松症
补肾中药
NOTCH信号通路
骨肉不相亲
Osteoporosis
Reinforcing the kidney
Notch signaling pathway
Flesh and blood blind date