响应面分析法优化超声辅助提取椪柑幼果中辛弗林的工艺

Optimization of Ultrasound Assisted Extration Technology for Synephrine from Young Fruits of Citrus poonensis Hort. ex Tanaka by Response Surface Methodology

研究采用超声波辅助提取椪柑幼果中辛弗林的提取工艺。在单因素试验基础上,选择影响提取效果的甲醇体积分数、超声温度、超声时间和液料比4个关键因素,以辛弗林得率为响应值,根据中心组合试验设计原理,采用四因素五水平的试验方案,模拟得到辛弗林得率的二次多项式回归方程的预测模型,得到超声辅助提取椪柑幼果中的辛弗林最佳条件:甲醇体积分数70.46%,超声温度34.45℃,超声时间45 min,液料比24.84 m L/g。在该条件下椪柑幼果中辛弗林得率的预测值为10.8769 mg/g DW,试验测定值为10.8463 mg/g DW,与回归方程的理论预测值相吻合。

An efficient ultrasound assisted extration technology was developed to extract synephrine from young fruits of Citrus poonensis Hort. ex Tanaka. A series of single-factor experiments were made. Based on the experiment results, a response surface design was generated by the Design-Expert software according to the Central Composite Design and ex- perimental data obtained were subjected to quadratic regression analysis. The results showed that the optimum extraction conditions were as follows： methanol concentration 70.46%, ultrasonic extraction temperature 34.45 ℃, ultrasonic extrac- tion time 45 min, liquid to solid ratio 24.84 mL/g. The predictive maximal yield of synephrine was 10.8769 mg/g DW. Validation experiments were carried out at these optimum conditions and the results indicated that the synephrine yield was 10.8463 mg/g DW. The results were basically correspond to model prediction.