腹水传代Walker256乳腺癌细胞系建立骨癌痛模型的可行性

The Feasibility of Ascites Passaged Walker256 Breast Cancer Cell Lines to Establish the Model of Bone Cancer Pain

目的：探讨应用腹水传代培养Walker256大鼠乳腺癌细胞系建立wistar大鼠胫骨癌痛模型的可行性。方法：将体重180．200gWistar大鼠随机分为三组：正常对照组（Control组）、假手术组（Fake组）、接种Walker256乳腺癌细胞组（Model组）。Model组为将含1×10^8个／mLWalker256大鼠乳腺癌细胞悬液20μL注入Wistar大鼠胫骨上段骨髓腔制备的骨癌症疼痛模型。Fake组经微量进样器注射等量的生理盐水入骨髓腔；Control组则不进行手术接种，分别于手术后数天（post-cancer cell implantation day，PID）PID0d、7d、14d及21d摄片检查手术侧胫骨，观察大鼠的疼痛行为学变化，PID0d、7d及14d行胫骨HE染色。结果：PID7d摄片检查提示骨密度不均一，HE染色见大量肿瘤细胞浸润、骨小梁破坏，PID14dModel组均与Fake组、Control组行为学方面有显著的统计学差异（P〈0．01）。结论：应用腹水传代培养Walker256大鼠乳腺癌细胞系可以建立wistar大鼠胫骨癌痛模型。

Objective： To investigate The possibility of ascites cultured Walker256 rat breast cancer cell line to establish the model of tibial cancer pain in Wistar rat. Methods： Wistar rats weighing 180-200g were randomly divided into three groups： normal control group （Control group）, sham group （Fake group）, cancer cells inoculated group （Model group）. In the model group, 20 μL Walker 256 breast cancer cell suspension with a cell density of 1×10^8/mL was injected into rat tibia bone marrow cavity. Fake animals were injected with an equal volume of saline; Control group did not undergo any surgical procedure. The X-ray examing, body weighting and pain behavior testing were carried on post-cancer cell implantation day （PID） 0, 7, 14 and 21, respectively. The tissue used for HE staining was removed on PID 0, 7 and 14. Results： In PID 7 d examination showed that the bone density was not uniform, and HE staining showed a large number of tumor cell infiltration and bone trabeeular destruction, in PID 14 d Model group, with Fake group and Control group had significant difference in pain behavior （P〈0.01）. Conclusion： A wistar rat model of bone cancer pain has been successfully established by using ascites cultured Walker 256 rat breast cancer cell line.