E-cad、IGF-1R 表达与肝癌患者肝切除术后复发的关系

The relationship between expression of E -cad and IGF -1R and the recurrence of patients with HCC after liver resection

目的：探讨 E-cad 和 IGF-1R 表达与原发性肝细胞癌（HCC）患者肝切除术后复发的关系。方法收集2010年1月至2012年12月因原发性 HCC 实施肝切除术的具备完整资料的91例连续病例，通过RT-PCR 方法测定组织标本中 E-cad 和 IGF-1R 表达，分别分析 E-cad 和 IGF-1R 的表达与临床病理因素以及肝切除术后复发的关系。结果 E-cad 在肝癌组织中的表达率较正常组织显著下降（38．7％比75．0％）。E-cad 阴性组患者其术后复发率明显增高（71．1％），其肿瘤侵及肝被膜者多（χ2＝5．144，P ＝0．036），TNM分期 Ⅱ ～Ⅳ期者多（χ2＝7．161，P ＝0．009）。IGF-1R 在肝癌组织中的表达率较正常组织显著上升（66．1％比20．0％），IGF-1R 阳性患者术后复发率显著增高（68．3％），其肿瘤侵及肝被膜者多（χ2＝5．144，P ＝0．036），无瘤包膜者多（χ2＝7．201，P ＝0．012），TNM分期 Ⅱ ～Ⅳ期者多（χ2＝4．195，P ＝0．014），门脉癌栓者多（χ2＝6．538，P ＝0．032）。E-cad 与 IGF-1R 表达存在显著负相关关系（χ2＝14．329，P ＝0．000）。结论 E-cad 在肝癌组织中表达显著低于正常肝组织。IGF-1R 在肝癌组织中表达显著高于正常组织。两者表达均与肝癌侵袭转移及患者术后复发相关。同时 E-cad 与 IGF-1R 在肝癌组织标本中的表达存在相关性，两者可能在肝癌发生发展中起协调作用。

Objective To investigate the relationship between expression of E -cad and IGF -1R and the recurrence of patients with primary hepatocellular carcinoma （HCC）who underwent liver resection.Methods The clinical data of 91 cases of primary HCC underwent liver resection from January 2010 to December 2012 were analyzed retrospectively.The expression of E -cad and IGF -1R was detected by RT -PCR,and the relationship between expression of E -cad and IGF -1R and the recurrence of patients was analyzed.Results The expression of E -cad was obviously lower in HCC tissues than that in normal liver tissues,with the expression percentage of 38.7% vs. 75.0%.The recurrence rate of E -cad negative group was higher（71.1%）.The TNM Ⅱ -Ⅳ stage （χ2 =7.161, P =0.009）and liver capsule invasion （χ2 =5.144,P =0.036）in E -cad negative group were higher than E -cad positive group.The expression of IGF -1R was obviously higher in HCC tissues than that in normal liver tissues,with the expression percentage of 66.1% vs.20.0%.The recurrence rate of IGF -1R negative group was higher（68.3%）. The TNMⅡ-Ⅳ stage （χ2 =4.195,P =0.014）,liver capsule invasion （χ2 =5.144,P =0.036）,non -tumor capsularin （χ2 =7.201,P =0.012）and PVTT（χ2 =6.538,P =0.032）in E -cad negative group were higher than E -cad positive group.And there was negative correlation between the expression of E -cad and IGF -1R（χ2 =14.329,P =0.000）.Conclusion The mRNA expression of E -cad in the tissues of HCC is obviously lower than normal liver tissues.The mRNA expression of IGF -1R in the tissues of HCC is obviously higher than normal liver tissues.The expressions of E -cad and IGF -1R are associated with the degree of local invasion and malignant.The E -cad and IGF -1R expression is correlated in the tissues of HCC,which means that E -cad and IGF -1R play synergistic effect in HCC genesis.