miR-181c-3p和miR-5692b在食管癌患者中的表达水平及其临床意义

Expression levels of miR-181c-3p and miR-5692b in esophageal cancer and their clinical significance

目的探讨miR-181c-3p和miR．5692b在食管癌患者中的表达水平及临床意义。方法应用基因芯片技术对食管癌组织的特异性microRNA进行筛查，然后利用即时荧光定量PCR技术对55例食管癌及癌旁对照组织的miR-181c．3p和miR-5692b表达情况进行检测。结果（1）癌组织中miR-181c．3p与miR-5692b的表达水平均较正常组织升高。（2）miR181c-3p和miR-5692b表达水平均与肿瘤大小、浸润深度及临床分期相关（均P〈0．05）。（3）miR-181c-3p和miR-5692b高表达组总生存率明显低于低表达组（均P〈0．05）。（4）多元Cox回归分析结果表明高表达miR-181c-3p和miR-5692b均为食管癌的预后不良因素。结论miR-181c-3p和miR-5692b的表达与食管癌的临床病理特征及预后存在显著相关性，可作为食管癌进展和预后的潜在预测指标。

Objective To study the expression level and clinical significance of miR-181c-3p and miR-5692b in esophageal cancer. Methods The microRNA （miRNA） profiles of esophageal squamous cell carcinoma were analyzed by miRNA microarray in 55 cases of esophageal cancer. The expression levels of miR-181c-3p and miR-5692b from 55 pairs of tumor tissues and adjacent non-neoplastic tissues were determined by qRT-PCR analysis. Results Both miR-181c-3p and miR-5692b were significantly up- regulated in tumor tissues compared with adjacent non-neoplastic tissues. Their expression was also significantly associated with tumor size, depth of invasion and clinical tumor stage （ P 〈 0. 05 ） . High expression of miR-181c-3p and miR-5692b were significantly associated with poor prognosis （P 〈 0. 05 ）. Multivariate Cox regression analysis confirmed that high expression of miR-181c-3p and miR-5692b was poor prognostic indicators in esophageal cancer. Conclusions There are significant correlation between miR-181c-3p/miR-5692b expression, clinicopathologic parameters and prognosis. They represent potential prognostic biomarkers in esophageal squamous cell carcinoma.