摘要
Engineering complex nanocomposites that specifically target the hepatitis B virus （HBV） and overcome the limitations of current therapies such as limited efficacy and serious side effects is very challenging. Here, for the first time, the antiviral effect of engineered plasmonic gold and layered double hydroxide self-assemblies （AuNPs/LDHs） is demonstrated, using HBV as a model virus and hepatoma-derived HepG2.2.215 ceils for viral replication, assembly, and secretion of infectious virions and subviral particles. AuNPs/LDHs were obtained by a simple, cost-effective procedure in which small AuNPs （-3.5 nm） were directly obtained and organized on the surface of larger LDH nanoparticles （-150 nm） by exploiting the capability of MgLDH, ZnLDH, and MgFeLDH to manifest their ＂structural memory＂ in the aqueous solution of Au（O2CCH3）3. The self-assembly approach of AuNPs and LDHs was assessed by transmission electron microscopy （TEM）, X-ray photoelectron spectroscopy （XPS）, powder X-ray diffraction （PXRD）, and UV-Vis analysis （UV-Vis）. All AuNPs/LDHs tested reduced the amount of viral and subviral particles released from treated cells by up to 80% and exhibited good cytocompatibility. AuNPs/MgFeLDH showed the highest antiviral HBV response with more than 90% inhibition of HBV secretion for the whole concentration range. Preliminary studies on the mechanism of HBV inhibition reveals that in the presence of AuNPs/LDHs, HBV particles are sequestered within the treated cells. The antiviral and low cytotoxic plasmonic properties of these Au/LDH nanocomposites indicate that they hold significant potential to be tailored as novel efficient therapeutics for the treatment of hepatitis B.
工程建筑群 nanocomposites 明确地指向肝炎 B 病毒(HBV ) 并且克服象有限功效和严肃的副作用那样的治疗很正在质问的电流的限制。第一次，这里，设计 plasmonic 黄金和分层的双氢氧化物 self-assemblies (AuNPs/LDHs ) 的抗病毒的效果被表明，用象为传染 virions 和 subviral 粒子的病毒的复制，汇编，和分泌物的一个模型病毒和导出 hepatoma 的 HepG2.2.215 房间的 HBV。 AuNPs/LDHs 被在小 AuNPs 的一个简单、划算的过程获得(~ 3.5 nm )直接在更大的 LDH nanoparticles 的表面上被获得并且组织(~ 150 nm )由利用 MgLDH 的能力， ZnLDH ，并且在 Au 的水的解决方案表明他们的结构的存储器的 MgFeLDH ( O <sub>2</sub > CCH <sub>3</sub>)<sub>3</sub>。自己组装 AuNPs 和 LDH 的途径被传播估计电子显微镜学(TEM ) ， X 光检查光电子光谱学(XPS ) ，粉末 X 光检查衍射(PXRD ) ，和 UVVis 分析(UVVis ) 。测试的所有 AuNPs/LDHs 减少了数量病毒并且多达 80% 免除对待的房间并且展出了好 cytocompatibility 的 subviral 粒子。AuNPs/MgFeLDH 为整个集中范围与 HBV 分泌物的超过 90% 抑制显示出最高抗病毒的 HBV 反应。HBV 抑制的机制上的初步的研究表明面对 AuNPs/LDHs， HBV 粒子在对待的房间以内被扣押。这些 Au/LDH nanocomposites 的抗病毒、低的细胞毒素的 plasmonic 性质显示他们保持重要潜力为肝炎 B 的处理作为新奇有效治疗学被定制。
