siRNA沉默YAP1对宫颈癌Hela细胞增殖和侵袭的影响

Effect of YAP1 expression down- regulated by siRNA interference on cell proliferation and invasion of human cervical cancer Hela cells

目的:用小干扰RNA(siRNA)沉默宫颈癌Hela细胞中YAP1基因的表达,观察YAP1表达降低对细胞增殖和侵袭能力的影响,初步探讨YAP1在宫颈癌中的作用。方法:qRT-PCR和Western blot方法检测宫颈癌Hela细胞及正常宫颈上皮细胞H8中YAP1的表达,siRNA YAP1转染Hela细胞沉默YAP1表达,并以转染control siRNA的细胞作为对照,转染48h后,分别用qRT-PCR和Western blot方法检测沉默效果。用MTT法检测沉默后细胞增殖情况。Transwell检测细胞侵袭能力。结果:YAP1在宫颈癌细胞中高表达。siRNA能够有效沉默Hela细胞中YAP1的mRNA及蛋白的表达。沉默YAP1表达能够明显抑制Hela细胞的增殖及侵袭能力。结论:YAP1蛋白在宫颈癌细胞中高表达并具有促进细胞增殖及侵袭的能力。

Objective： To investigate the influence of the expression of Yes- associated protein 1（ YAP1） down-regulated by siRNA interference on the cell proliferation and invasion of human cervical cancer cells. Methods： Detect the expression of YAP1 in cervical cancer cells and cervical epithelial cells by qRT- PCR and Western blot. The cell proliferation rate of Hela cells was detected by MTT assay to evaluate the effect of silencing YAP1 on proliferation of Hela cells. Transwell experimental was used to detect the invasion ability of Hela cells. Results： Compared Hela cells with H8 cells,YAP1 expression in Hela cells were significantly higher than H8 cells. Compared with the control siRNA group,the proliferation and invasion ability of siRNA group cells decreased significantly. Conclusion： YAP1 was high expressed in Hela cells and promote cells proliferation and invasion ability.