三阴性乳腺癌细胞免疫状态和Th1/Th2细胞因子的变化

Cellullar immune state and the change of Th1/Th2 cytokines in the triple negative breast cancer

目的:探讨三阴性乳腺癌的细胞免疫状态和Th1/Th2细胞因子的变化。方法:比较46例三阴性乳腺癌患者和60例非三阴性乳腺癌患者的临床资料,分析三阴性乳腺癌组、非三阴性乳腺癌组和对照组的细胞免疫状态及Th1/Th2细胞因子的变化。结果:三阴性乳腺癌和非三阴性乳腺癌两组相比,在发病年龄、月经状况、临床分期、组织学分级情况没有统计学差异(P>0.05);在淋巴结转移和内脏转移方面有统计学差异(P<0.05)。乳腺癌患者CD3、CD4、CD19 T细胞亚群无改变,CD8和CD69水平有变化,但后两者在三阴性乳腺癌和非三阴性乳腺癌组中没有统计学差异(P>0.05)。乳腺癌患者IL-2分泌减少,IL-6、IL-10分泌高,存在Th2漂移。结论:三阴性乳腺癌转移风险高,预后差。乳腺癌存在Th1/Th2失衡,但三阴性乳腺癌和非三阴性乳腺癌相比,并没有明显的Th2优势,说明三阴性乳腺癌发病机制具有更为复杂的生物学因素。

Objective： To investigate cellular immune state and the change of Th1 /Th2 cytokines. Methods： Clinicopathological data were compared between the 46 cases of triple negative breast cancer（ TNBC） and 60 cases of non- triple negative breast cancer（ non- TNBC）. Cellular immune state and the change of Th1 / Th2 cytokines were analyzed with 40 cases of control group,TNBC patients and non- TNBC patients. Results： There had not significant differences in disease onset,menstrual status,clinical stages,tumor histologic grade（ P〉0. 05）,but had significant differences in lymph note positivity and metastatic status between TNBC and non- TNBC patients（ P〈0. 05）. The expression of CD3,CD4,CD19 had no significant change compared with control group,the variety of CD8 and CD69 had no differences between the TNBC patients and non- TNBC（ P〉0. 05）. IL- 6,IL- 10 expression levels in breast cancer was higher than that in healthy control group（ P〈0. 05）,but IL- 2 expression level was lower,the balance between Th1 and Th2 shifts to the direction of Th2. Conclusion： TNBC has high risk of metastasis and poor prognosis.The unbalance of Th1 / Th2 was obvious in breast cancer,but there is not significant Th2 advance in TNBC. It indicates more complex biological factors in TNBC pathogenesis.