摘要
铂 nanoparticles (NP ) 被报导模仿各种各样的抗氧化剂酶并且可以因此由减少反应的氧生产积极生物效果种类(ROS ) 层次。在这张手稿，我们报导磅 NP 由在金 nanorods (Au@Pt NDR ) 上扔铂 nanodots ferroxidase 作为酶模仿。Au@Pt NDR 显示出 pH 依赖的象 ferroxidase 一样活动并且在中立 pH 价值举办更高的活动。有人的房间线(肺腺癌 A549 和正常支气管的上皮的房间线 HBE ) 的 Cytotoxicity 结果证明 Au@Pt NDR 经由 endocytosis 和 translocate 被收起进房间进 endosome/lysosome。Au@Pt NDR 在比 0.15 n 低的 NDR 粒子集中有好 biocompatibility。然而，面对 H <sub>2</sub > O <sub>2</sub>, lysosomelocated NDR 展出象 peroxidase 一样活动因此增加 cytotoxicity。面对 Fe <sup>2+</sup>, ， NDR 的象 ferroxidase 一样活动由消费 H <sub>2</sub 保护房间免受氧化应力的伤害 > O <sub>2</sub> 。当在生物系统采用 Au@Pt NDR 时，彻底的考虑应该被给这行为。
Platinum nanoparticles （NPs） are reported to mimic various anfioxidant enzymes and thus may produce a positive biological effect by reducing reactive oxygen species （ROS） levels. In this manuscript, we report Pt NPs as an enzyme mimic of ferroxidase by depositing platinum nanodots on gold nanorods （Au@Pt NDRs）. Au@Pt NDRs show pH-dependent ferroxidase-like activity and have higher activity at neutral pH values. Cytotoxicity results with human cell lines （lung adenocarcinoma A549 and normal bronchial epithelial cell line HBE） show that Au@Pt NDRs are taken up into cells via endocytosis and translocate into the endosome/lysosome. Au@Pt NDRs have good biocompatibility at NDR particle concentrations lower than 0.15 nM. However, in the presence of H202, lysosome- located NDRs exhibit peroxidase-like activity and therefore increase cytotoxicity. In the presence of FeE＋, the ferroxidase-like activity of the NDRs protects cells from oxidative stress by consuming H202. Thorough consideration should be given to this behavior when employinK Au@Pt NDRs in biological svstems.
