摘要
为提高非水相脂肪酶法拆分DL-薄荷醇反应的底物浓度,建立了以乙酸乙烯酯为酰基供体的无溶剂体系。实验考察了底物浓度、酶量、温度和反应时间等因素对脂肪酶Lipozyme TL IM动力学拆分DL-薄荷醇的影响,得到最佳反应条件为DL-薄荷醇浓度2.8 mol/L,酶用量0.5 g,反应温度30℃,反应时间6 h。此条件下,反应转化率为22.7%,e.e.p为99.1%。脂肪酶Lipozyme TL IM重复利用20次,酶立体选择性基本不变,相对酶活为38.7%。
In order to enhance the substrate concentration of lipase catalyzed resolution of DL-menthol in non-aqueous phase,a solvent-free system was established using vinyl acetate as acyl donor.The effects of substrate concentration,enzyme dosage,temperature and reaction time on kinetic resolution of DL-menthol were investigated.The optimum conditions were determined as follows：substrate concentration 2.8 mol/L,enzyme dosage 0.5 g,reaction temperature 30 ℃,reaction time 6 h.Under above conditions,the conversion reached 22.7% with e.e.pvalue of 99.1%.In addition,the lipase reserved 38.7% of its activity with stable enantioselectivity after 20 recycles
出处
《发酵科技通讯》
CAS
2015年第4期15-18,共4页
Bulletin of Fermentation Science and Technology
