Rho激酶在房颤患者左心耳中的表达

Expression of Rho associated coil forming protein kinase in left atrial appendage of patients with atrial fibrillation

目的研究Rho激酶(ROCK-1)及其底物—肌球蛋白磷酸酶靶亚基1(MYPT-1)在房颤患者左心耳中的表达,探讨Rock信号转导通路在房颤发生中的作用。方法 40例接受开胸手术者分为房颤组和窦性心律组,手术中取约100 mg左心耳组织。采用免疫组化ABC法检测两组左心耳组织的蛋白表达水平。结果 ROCK-1和MYPT-1在房颤患者左心耳中表达较对照组明显上调,差异有统计学意义(P<0.01)。房颤组ROCK-1和MYPT-1的蛋白表达,二者呈显著正相关(r=0.981,P<0.01)。结论房颤患者左心耳组织中明显存在ROCK-1表达上调与功能活化,提示Rho激酶信号转导通路在房颤病变的发生机制中具有重要作用。

Objective To detect the expressions of Rho associated coil forming protein kinase - 1 （ ROCK - 1 ） and its substrate myosin phosphatase target subunit - 1 （ MYPT - 1 ） in left atrial appendage of patients with atrial fibrillation （AF）. Methods Left atrial appendage tis- sue samples of 40 patients undergoing cardiac surgery were examined. 20 patients had AF, 20 patients had no history of AF. The expressions of ROCK - 1 and MYPT - 1 in left atrial appendage were detected by immunohistochemistry. Results Immunohistochemistry showed that the expres- sions of ROCK - 1 and MYPT - 1 were significantly up - regulated in left atrial appendage of AF patients （ P 〈0. O1 ）. The two indicators were positively correlated （ r =0. 981, P 〈 0.01 ）. Conclusion The ROCK - 1 expression in left atrial appendage of patients with AF is up - regula- ted and activated, which implies that ROCK may play a significant role in the pathological changes in AF.