肝癌中Oct4蛋白的表达及与临床病理因素的关系

Study on the relationship between expression of Oct4 protein with clinical pathological factors in patients with hepatocellular carcinoma

目的检测八聚体结合转录因子4(Oct4)蛋白在肝细胞癌组织中的表达,并探讨其与临床病理因素的相关性。方法收集60例肝癌患者手术切除的肝细胞癌组织及对应的癌旁组织标本,应用免疫组织化学方法检测Oct4蛋白的表达,观察Oct4蛋白表达与临床病理因素的关系。结果肝细胞癌组织Oct4蛋白阳性表达率显著高于癌旁组织(81.67%vs.15.0%)(P<0.05);肝细胞癌组织中Oct4蛋白的表达与肿瘤大小、血管侵犯及TNM分期有关(P<0.05)。结论 Oct4蛋白在肝细胞癌的发生发展中发挥重要作用,可作为判断预后有价值的分子标记物。

Objective To study the significance of expression of Oct4 protein in tissue of hepatocellular carcinoma, and to discuss its cor- relation with clinical pathologic factors. Methods A total of 60 tissue samples from patients with hepatocellular carcinoma and the corresponding tissue adjacent to carcinoma were collected for this study, immnohisochemical technique had been used to detect the expression of Oct4 protein. The relationship between clinical pathologic factors and expression of Oct4 protein had been studied. Results The positive expression rate of Octg protein was higher in tissue of hepatocellular carcinoma than that from the tumor adjacent tissue （75.9 % vs. 33.3 % ） （ P 〈 0.05 ）, it was posi- tively correlated with tumor size and closely related to its vascular invasion and TNM stages （ P 〈 0.05 ）. Conclusion Oct4 protein is associated with the differentiation of tumor in hepatocellular carcinoma and it may be taken as a valuable molecular marker for prediction of the prognosis of patients with hepatocellular carcinoma.