FLOT1蛋白在胃癌组织中的表达及临床意义

Clinical significance of flotillin-1 overexpression in human gastric cancer

目的:探讨浮舰蛋白-1(flotillin—1,FLOT1)在胃癌组织中的表达水平及其与预后相关的临床意义.方法:分别运用荧光定量PCR和Western blot方法检测16组新鲜胃癌及癌旁组织中FLOTI RNA和FLOT1蛋白的表达情况;采用免疫组织化学法检测187例胃癌及癌旁石蜡组织中FLOT1的表达水平.分析FLOT1蛋白的表达与胃癌患者临床病理特征及预后的关系.结果:FLOT1 RNA和FLOT1蛋白在新鲜胃癌组织中均呈高表达(13/16;12/16).FLOT1在胃癌组织中的阳性表达率高于癌旁正常组织(92.35%vs 26.67%,P<0.05);FLOT1蛋白的表达与胃癌患者的性别、年龄、肿瘤大小、肿瘤分化程度和肿瘤位置均无关(P>0.05),仅与肿瘤浸润深度(P=0.000),肿瘤淋巴结转移(P=0.000),肿瘤远处转移(P=0.000)和肿瘤AJCC临床分期(P=0.016)有关.FLOT1高表达预示患者预后较差.结论:FLOT1过表达可能与胃癌的侵袭转移及预后密切相关,有望成为胃癌的早期诊断指标和治疗靶点.

AIM：To investigate the expression of flotillin-1（FLOT1） protein in gastric cancer and analyze their correlation with pathological characteristics and prognosis.METHODS：We detected the FLOT1 expression in fresh gastric cancer samples and matched adjacent noncancerous tissue samples by real-time florescence quantitative PCR（qRTPCR）（n=16） and Western blot analysis（n=16）.Additionally,immunohistochemistry was performed to examine FLOT1 protein expression in paraffin-embedded gastric carcinoma tissues and matched normal specimens from 187 gastric cancer patients.Statistical analyses were applied to evaluate the associations of FLOT1 expression with clinical parameters and prognosis.RESULTS：FLOT1 mRNA and protein levels were evidently up-regulated in gastric cancer tissues compared with adjacent non-cancerous tissue samples（13/16;12/16）.FLOT1 expression in gastric cancer tissues was significantly higher than that in corresponding adjacent normal tissues（92.35%vs 26.67%,P〈0.05）.Expression of FLOT1 in tumor tissue was not significantly associated with gender,age,tumor size,differentiation status or tumor location.However,elevated FLOT1 expression was strongly correlated with depth of invasion（P=0.000）,lymph node metastasis（P=0.000）,distant metastasis（P=0.000）,and AJCC stage（P=0.016）.Elevated FLOT1 cases had a poor prognosis.CONCLUSION：FLOT1 expression is significantly correlated with depth of invasion and distant metastasis.FLOT1 may be used as an early diagnostic marker and therapeutic target for gastric carcinoma.