肠易激综合征患者肠黏膜VIPR、CGRPR的变化情况

Expression of colonic vasoactive intestinal peptide receptor and calcitonin generelated peptide receptor in irritable bowel syndrome

目的:探讨肠易激综合征(irritable bowel syndrome,IBS)患者结肠黏膜血管活性肠肽受体(vasoactive intestinal peptide receptor,VIPR)、降钙素基因相关肽受体(calcitonin gene-related peptide receptor,CGRPR)的变化,以及他们在IBS中的可能作用及临床意义.方法:对腹泻型IBS(IBS-D)20例、便秘型IBS(IBS-C)8例和正常对照组(control)8例各取乙状结肠黏膜标本,应用实时荧光定量PCR、免疫组织化学染色法分别检测VIPR,CGRPR.结果:IBS-D组VIPR mRNA表达水平显著高于IBS-C组和control组(2.89±1.74vs0.85±0.6、0.62±0.31,P<0.05),IBS-C组和control组比较差异无统计学意义;IBS-D组CGRPR mRNA表达水平显著高于control组.(1.86±1.36 vs 0.77±0.5,P<0.05),IBS-C组和IBS-D及control组比较差异无统计学意义;IBS-D、IBS-C组VIPR表达水平显著高于control组(0.24±0.03、0.17±0.02 vs 0.13±0.01,P<0.05),IBS-D、IBS-C组间有显著差异性(P<0.05);IBS-D、IBS-C组CGRPR表达水平显著高于control组(0.23±0.02、0.18±0.02 vs 0.13±0.02,P<0.05),IBS-D、IBS-C组间有显著差异性(P<0.05).结论:VIPR、CGRPR可能参与了IBS的病理生理过程.VIPR、CGRPR在肠黏膜中的作用对于进一步研究这些脑肠肽受体在IBS发病机制的病理生理意义具有一定意义.

AIM：To investigate the expression of vasoactive intestinal peptide receptor（VIPR） and calcitonin gene-related peptide receptor（CGRPR） in the colon mucosa of patients with irritable bowel syndrome（IBS）,and to study their possible roles.METHODS：Endoscopic biopsies of the sigmoid colon were collected from 20 patients with diarrhea-predominant IBS（IBS-D）,8 with constipation-predominant IBS（IBS-C）and8 healthy volunteers（controls）.The mRNA expression of VIPR and CGRPR was evaluated by qRT-PCR.The immunohistochemical method was conducted to detect the expression of VIPR and CGRPR proteins.The results of immunohistochemistry were analyzed with Image Pro plus 6.0.RESULTS：Elevation of the mRNA expression of VIPR was found in IBS-D patients compared with IBS-C patients and controls（2.89±1.74 vs 0.85±0.6,0.62±0.31,P〈0.05）.No significant difference was observed between IBS-C patients and controls.The expression of CGRPR mRNA was elevated in IBS-D patients compared with controls（1.86±1.36 vs 0.77±0.5,P〈0.05）,but no significant difference was observed between IBS-C and IBS-D/ controls.Compared with healthy controls,significant up-regulation of VIPR and CGRPR was found in IBS-D and IBS-C patients（VIPR：0.24±0.03,0.17±0.02 vs 0.13±0.01,P〈0.05;CGRPR：0.23±0.02,0.18±0.02 vs 0.13±0.02,P〈0.05）,and a significant difference was also observed between IBS-D and IBS-C patients（P〈0.05）.CONCLUSION：VIPR and CGRPR are involved in the patho physiology of IBS in certain ways.The possible roles of VIPR and CGRPR in the colon suggest that further studies of the alterations of these neuropeptide receptors may be useful in understanding IBS pathophysiology.